Human IgD Matched Antibody Pair [RM123/RM129], 31-1025-MK

Uniprot: P01880
Gene ID: 3495
Host Species: Rabbit
Species Reactivity: Human
Concentration: 1) Capture Antibody: Anti-Human IgG4 Rabbit Monoclonal antibody, clone RM120, 100 ug at 1.0 mg/mL in 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide; 2) Detection Antibody: Biotin Anti-Human Ig Light Chain Rabbit Monoclonal Antibody, Clone RM129, 25 ug at 1.0 mg/mL in 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide; 3) 10X Sample Diluent: Added to the tested sample to reduce non-specific background and noise

CAT.NO. : 31-1025--MK

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Size:

Capture:100μg;Detection:25μg;Diluent:10mL;

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Product Details


Product Name	Human IgD Matched Antibody Pair [RM123/RM129]
Product Description	The Human IgD Matched Antibody Pairs is specific to Human IgD and has been demonstrated through ELISA. It does not cross-react to IgG from Non-Human Primate species (Rhesus and Cynomologus macaques), or mouse, rat or goat immunoglobulin. The pair can be used for the detection of therapeutic antibodies from samples obtained from experimental animals to provide accurate pharmacokinetic (PK) data. This product includes capture and detection antibodies and sample diluent buffer.
Catalog No.	31-1025-MK
Clone Name	RM123 Anti-Human IgD Rabbit Monoclonal Antibody RM129 Biotin Anti-Human Ig Light Chain Rabbit Monoclonal Antibody
Product Data Sheet	Human IgD Matched Antibody Pair
Protocol	Sandwich ELISA Protocol
Specificity	The antibody pair detects only human IgD. It does not react to monkey (Cyno or Rhesus) IgG, mouse IgG, rat IgG, or goat IgG. There is no cross reactivity to any other immunoglobulins or IgG subclasses.
Application	Sandwich ELISA, or other Sandwich Assays
Species Reactivity	Human
Conjugate	RM129: Biotin
Intended Use	Research Use Only
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Properties

Form	Liquid
Storage Instructions	Store at -20.0°C
Stability	Stable for 1 Year at -20.0°C from date of receipt
Components	1)Capture Antibody: Anti-Human IgD Rabbit Monoclonal antibody, clone RM123, 100 µg at 1.0 mg/mL in 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide; 2)Detection Antibody: Biotin Anti-Human Ig Light Chain Rabbit Monoclonal Antibody, Clone RM129, 25 µg at 1.0 mg/mL in 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide; 3)10X Sample Diluent: Added to the tested sample to reduce non-specific background and noise
Purity	Protein A affinity purified from an animal origin–free culture supernatant
Clone Type	Monoclonal
Isotype	Rabbit IgG
Keywords
Usage	ELISA: Capture Antibody 50 ng/well – 200 ng/well; Detection Antibody 0.1 µg/mL – 0.5 µg/mL
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Product Images

	Sandwich ELISA using RM123 as the capture antibody (100 ng/well), and Biotinylated anti-human light chains (k+l) antibody RM129 (0.2 µg/mL) as the detection antibody, followed by an alkaline phosphatase conjugated streptavidin.
	Sandwich ELISA, using RevMAb human IgD matched antibody pair (capture: RM123; detection: Biotin-RM129), shows species reactivity to human only, and shows no cross-reactivity to monkey (Cyno or Rhesus), mouse IgD, rat IgD, or goat IgD.
	Sandwich ELISA, using RevMAb human IgD matched antibody pair, shows no cross- reactivity with Human IgG, IgE, IgA, or IgM. The plate was coated with human IgD capture antibody (50ng/well). Different immunoglobulin samples (0.2 µg/mL) were added and biotinylated RM129 (0.2 µg/mL) was used as detection antibody, followed by an alkaline phosphatase conjugated streptavidin

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