Human IGFBP1 ELISA Kit
Key features and details
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- 反应物种:
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- 偶联标记说明:
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CAT.NO. : ARG80157
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Product Details
内含物
| 货号 | 内含物名称 | 包装 | 温度 |
|---|---|---|---|
| ARG80157-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG80157-002 | Standard (Lyophilized) | 5 X 0.75 ng/vial | 4°C |
| ARG80157-003 | Standard diluent buffer | 20 ml | 4°C |
| ARG80157-004 | Antibody conjugate concentrate | 400 µl | 4°C |
| ARG80157-005 | Antibody diluent buffer | 16 ml | 4°C |
| ARG80157-006 | HRP-Streptavidin concentrate | 400 µl | 4°C (Protect from light) |
| ARG80157-007 | HRP-Streptavidin diluent buffer | 16 ml | 4°C |
| ARG80157-008 | 20X Wash buffer | 50 ml | 4°C |
| ARG80157-009 | TMB substrate | 12ml | 4°C (Protect from light) |
| ARG80157-010 | STOP solution | 12ml | 4°C |
| ARG80157-011 | Plate sealer | 4 strips | Room temperature |
概述
| 产品描述 | ARG80157 Human IGFBP1 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human IGFBP1 in Serum, Plasma, Cell culture supernatants. |
|---|---|
| 反应物种 | Hu |
| 应用 | ELISA |
| 靶点名称 | IGFBP1 |
| 偶联标记 | HRP |
| 偶联标记说明 | Substrate: TMB and read at 450 nm |
| 灵敏度 | 25 pg/ml |
| 样品类型 | Serum, Plasma, Cell culture supernatants |
| 标准范围 | 46.8 - 3000 pg/ml |
| 样本量 | 100 µl |
| 精确 | CV: less than10% |
| 別名 | IBP-1; IBP1; PP12; IGF-BP25; Insulin-like growth factor-binding protein 1; hIGFBP-1; IGFBP-1; Placental protein 12; AFBP; IGF-binding protein 1 |
应用说明
| 检测时间 | 4 hours |
|---|
属性
| 形式 | 96 well |
|---|---|
| 存放说明 | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | Swiss-port # P08833 Human Insulin-like growth factor-binding protein 1 |
|---|---|
| 基因名称 | IGFBP1 |
| 全名 | insulin-like growth factor binding protein 1 |
| 背景介绍 | This gene is a member of the insulin-like growth factor binding protein (IGFBP) family and encodes a protein with an IGFBP domain and a thyroglobulin type-I domain. The protein binds both insulin-like growth factors (IGFs) I and II and circulates in the plasma. Binding of this protein prolongs the half-life of the IGFs and alters their interaction with cell surface receptors. [provided by RefSeq, Jul 2008] |
| 生物功能 | IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. Promotes cell migration. [UniProt] |
| 产品亮点 | Related products: IGFBP1 antibodies; IGFBP1 ELISA Kits; Related news: Detecting MMPs and their non-ECM substrates New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| 研究领域 | Cell Biology and Cellular Response kit; Signaling Transduction kit |
| 翻译后修饰 | Phosphorylated; probably by casein kinase II. Phosphorylation alters the affinity of the protein for IGFs. In amniotic fluid, the unmodified protein is the most abundant form, while mono-, bi-, tri- and tetraphosphorylated forms are present in decreasing amounts. The phosphorylation state may influence the propensity to proteolysis. |
检测图片 (1)
ARG80157 Human IGF-BP1 ELISA Kit standard curve image
ARG80157 Human IGF-BP1 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
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