Human IgG3 Matched Antibody Pair [RM119/RM129], 31-1021-MK

Key features and details

  • Uniprot: P01860
  • Gene ID: 3502
  • Host Species: Rabbit
  • Species Reactivity: Human
  • Concentration: 1) Capture Antibody: Anti-Human IgG3 Rabbit Monoclonal antibody, clone RM119, 100 ug at 1.0 mg/mL in 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide; 2) Detection Antibody: Biotin Anti-Human Ig Light Chain Rabbit Monoclonal Antibody, Clone RM129, 25 ug at 1.0 mg/mL in 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide; 3) 10X Sample Diluent: Added to the tested sample to reduce non-specific background and noise
  • Brand:
CAT.NO. : 31-1021-MK
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Product Details
Product Details
Product NameHuman IgG3 Matched Antibody Pair [RM119/RM129]

Rabbit Monoclonal
Product DescriptionThe Human IgG3 Matched Antibody Pairs is specific to Human IgG3 and has been demonstrated through ELISA to not recognize (cross) IgG from Non-Human Primate species (Rhesus and Cynomologus macaques), nor does the matched pair recognize mouse, rat or goat immunoglobulin. The kit can be used for the detection of therapeutic antibodies from samples obtained from experimental animals to provide accurate pharmacokinetic (PK) data.
Catalog No.31-1021-MK
Clone NameRM119 Anti-Human IgG3 Rabbit Monoclonal Antibody

RM129 Biotin Anti-Human Ig Light Chain Rabbit Monoclonal Antibody
Product Data SheetHuman IgG3 Matched Antibody Pair
ProtocolSandwich ELISA Protocol
SpecificityThe antibody pair detects only human IgG3. It does not react to monkey (Cyno or Rhesus) IgG, mouse IgG, rat IgG, or goat IgG. There is no cross reactivity to any other immunoglobulins or IgG subclasses.
ApplicationSandwich ELISA, or other Sandwich Assays
Species ReactivityHuman
ConjugateRM129: Biotin
Intended UseResearch Use Only
.
Properties
FormLiquid
Storage InstructionsStore at -20.0°C
StabilityStable for 1 Year at -20.0°C from date of receipt
Components1)Capture Antibody: Anti-Human IgG3 Rabbit Monoclonal antibody, clone RM119, 100 µg at 1.0 mg/mL in 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide;
2)Detection Antibody: Biotin Anti-Human Ig Light Chain Rabbit Monoclonal Antibody, Clone RM129, 25 µg at 1.0 mg/mL in 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide;
3)10X Sample Diluent: Added to the tested sample to reduce non-specific background and noise
PurityProtein A affinity purified from an animal origin–free culture supernatant
Clone TypeMonoclonal
IsotypeRabbit IgG
Keywords
UsageELISA: Capture Antibody 50 ng/well – 200 ng/well;
Detection Antibody 0.1 µg/mL – 0.5 µg/mL
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Product Images
Sandwich

Sandwich ELISA, using RevMAb human IgG3 matched antibody pair, shows species reactivity to human only, and shows no cross-reactivity to monkey (Cyno or Rhesus), mouse IgG, rat IgG, or goat IgG.

Detection

Detection of human IgG3 in monkey serum. Sandwich ELISA using RevMAb human IgG3 matched antibody pair, followed by a HRP conjugated streptavidin.

Detection

Detection of human IgG3 in mouse or rat serum. Sandwich ELISA using RevMAb human IgG3 matched antibody pair, followed by a HRP conjugated streptavidin.

Detection

Detection of human IgG3 in mouse or rat serum. Sandwich ELISA using RevMAb human IgG3 matched antibody pair, followed by a HRP conjugated streptavidin.

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