Human IgM Matched Antibody Pair [RM121/RM129], 31-1023-MK

Key features and details

  • Uniprot: P01871
  • Gene ID: 3507
  • Host Species: Rabbit
  • Species Reactivity: Human
  • Concentration: 1) Capture Antibody: Anti-Human IgG4 Rabbit Monoclonal antibody, clone RM120, 100 ug at 1.0 mg/mL in 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide; 2) Detection Antibody: Biotin Anti-Human Ig Light Chain Rabbit Monoclonal Antibody, Clone RM129, 25 ug at 1.0 mg/mL in 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide; 3) 10X Sample Diluent: Added to the tested sample to reduce non-specific background and noise
  • Brand:
CAT.NO. : 31-1023-MK
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Product Details
Product Details
Product NameHuman IgM Matched Antibody Pair [RM121/RM129]
Product DescriptionThe Human IgM Matched Antibody Pairs is specific to Human IgM and has been demonstrated through ELISA. It does not cross-react to IgG from Non-Human Primate species (Rhesus and Cynomologus macaques), or mouse, rat or goat immunoglobulin. The pair can be used for the detection of therapeutic antibodies from samples obtained from experimental animals to provide accurate pharmacokinetic (PK) data. This product includes capture and detection antibodies and sample diluent buffer.
Catalog No.31-1023-MK
Clone NameRM121 Anti-Human IgM Rabbit Monoclonal Antibody

RM129 Biotin Anti-Human Ig Light Chain Rabbit Monoclonal Antibody
Product Data SheetHuman IgM Matched Antibody Pair
ProtocolSandwich ELISA Protocol
SpecificityThe antibody pair detects only human IgM. It does not react to monkey (Cyno or Rhesus) IgG, mouse IgG, rat IgG, or goat IgG. There is no cross reactivity to any other immunoglobulins or IgG subclasses.
ApplicationSandwich ELISA, or other Sandwich Assays
Species ReactivityHuman
ConjugateRM129: Biotin
Intended UseResearch Use Only
.
Properties
FormLiquid
Storage InstructionsStore at -20.0°C
StabilityStable for 1 Year at -20.0°C from date of receipt
Components1)Capture Antibody: Anti-Human IgM Rabbit Monoclonal antibody, clone RM121, 100 µg at 1.0 mg/mL in 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide;
2)Detection Antibody: Biotin Anti-Human Ig Light Chain Rabbit Monoclonal Antibody, Clone RM129, 25 µg at 1.0 mg/mL in 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide;
3)10X Sample Diluent: Added to the tested sample to reduce non-specific background and noise
PurityProtein A affinity purified from an animal origin–free culture supernatant
Clone TypeMonoclonal
IsotypeRabbit IgG
Keywords
UsageELISA: Capture Antibody 50 ng/well – 200 ng/well;
Detection Antibody 0.1 µg/mL – 0.5 µg/mL
.
Product Images
Sandwich

Sandwich ELISA using RM121 as the capture
antibody (100 ng/well), and Biotinylated anti-human light
chains (k+l) antibody RM129 (0.2 µg/mL) as the detection
antibody, followed by an alkaline phosphatase
conjugated streptavidin.

.

Sandwich ELISA, using RevMAb human IgM matched
antibody pair (capture: RM121; detection: Biotin-RM129),
shows species reactivity to human IgM only, and shows
no cross-reactivity to monkey (Cyno or Rhesus),
mouse IgM, rat IgM, or goat.

Sandwich

Sandwich ELISA, using RevMAb human IgM matched antibody
pair, shows no cross- reactivity with Human IgG, IgE, IgA, or IgD.
The plate was coated with human IgM capture antibody (50 ng/well).
Different immunoglobulin samples (0.2 µg/mL) were added
and biotinylated RM129 (0.2 µg/mL) was used as detection antibody,
followed by an alkaline phosphatase conjugated streptavidin.

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