Human CKMB ELISA Kit

Protocol Booklet

Key features and details

Specification: 96 Test
Sensitivity: 0.005 pg/ml (50 μl)；0.07 ng/ml (10 μl)
Standard Curve Range: 0.04~30 ng/ml
Standard Curve Gradient: 7 Points/3 Folds
Number of Incubations: 2
Sample Volume: 50 μl/10 μl
Assay type: Sandwich Elisa
Operation Duration: 120min

Brand:

CAT.NO. : AEH0072

RMB Please choose

Size:

96T

Trail, Bulk size or Custom requests Please contact us

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Product Details

Background

The creatine kinase molecule is a dimer composed of a brain-type subunit (B) and a muscle-type subunit (M). There are usually three isozymes in normal human tissues, which are CK-BB (CK1), CK-MB (CK2) and CK-MM (CK3) in the order of electrophoresis. CK-BB is mainly found in smooth muscle and brain tissue, and almost none in serum; CK-MB mainly exists in myocardial tissue, accounting for 0~4% of CK in serum; CK-MM is mainly present in skeletal muscle, accounting for 96%~100% of CK in serum. CK-MB has its own subtypes, including MB2 (tissue form) and MB1 (transformed form). After the release of CK-MB2 from the myocardium, the carboxy-terminus is hydrolyzed by plasma carboxypeptidase N, and a lysine residue is removed to be converted to the serum-modified isoform CK-MB1. CK and CK-MB are one of the sensitive indicators of myocardial tissue injury, especially CK-MB is more specific. The increase in CK during myocardial injury is mainly due to the increase in CK-MB, which is manifested by the simultaneous increase of both.

Typical data

ng/ml	O.D.		Average	Corrected
0.00	0.0144	0.0158	0.0151
0.04	0.0332	0.0309	0.0321	0.0170
0.12	0.0716	0.0726	0.0721	0.0570
0.37	0.1778	0.1780	0.1779	0.1628
1.11	0.4794	0.4464	0.4629	0.4478
3.33	1.3260	1.3850	1.3555	1.3404
10.00	3.2180	3.2230	3.2205	3.2054
30.00	4.5063	4.4600	4.4832	4.4681

Precision

	Intra-assay Precision			Inter-assay Precision
Sample Number	S1	S2	S3	S1	S2	S3
Sample Number	22	22	22	6	6	6
Average（ng/ml）	0.7	3.8	13.1	0.6	3.2	11.0
Standard deviation	0.1	0.2	1.0	0.1	0.1	0.3
Coefficient of variation（%）	5.6	4.6	7.3	3.6	3.9	3.1

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.

Spike Recovery

The spike recovery was evaluated by spiking 3 levels of human CKMB into health human serum sample. The un-spiked serum was used as blank in this experiment.

The recovery ranged from 79% to 119% with an overall mean recovery of 103%.

Sample Values

Sample Matrix

Sample

Evaluated

Range

(ng/ml)

Detectable

Mean of Detectable

(ng/ml)

Serum

n.d.-0.06

0.02

Serum/Plasma–Thirty samples from apparently healthy volunteers were evaluated for the presence of human CKMB in this assay. No medical histories were available for the donors.

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