Human IL-13 ELISA Kit
Protocol Booklet
Key features and details
- Specification:
- Sensitivity:
- Standard Curve Range:
- Standard Curve Gradient:
- Number of Incubations:
- Sample Volume:
- Assay type:
- Operation Duration:
-
Brand:
CAT.NO. : AEH0049
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Size:
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Product Details
Background
IL-13 (Interleukin-13) plays a key role in the pathogenesis of allergy, cancer, and tissue fibrosis. It is secreted by several helper T cell subsets, NK cells, mast cells, eosinophils, basophils, and visceral smooth muscle cells. IL-13 suppresses the production of proinflammatory cytokines and other cytotoxic substances by macrophages, fibroblasts, and endothelial cells. It promotes B cell activation, immunoglobulin class switching to IgE, and the upregulation of CD23/Fc epsilon RII. IL-13 signals through a receptor complex containing IL-13 R alpha 1 and IL-4 R alpha. This complex also functions as the type 2 IL-4 receptor. IL-13 R alpha 2 binds IL-13 and prevents IL-13 from signaling through the IL-13 R alpha 1/IL-4 R alpha complex. IL-13-bound IL-13 R alpha 2 can directly promote tumor cell invasiveness and the development of tissue fibrosis.
Typical data
| pg/ml | O.D. | Average | Corrected | |
| 0.00 | 0.0188 | 0.0241 | 0.0215 | |
| 312.5 | 0.0291 | 0.0295 | 0.0293 | 0.0079 |
| 625.00 | 0.0469 | 0.0458 | 0.0464 | 0.0249 |
| 1250.00 | 0.0932 | 0.0879 | 0.0906 | 0.0691 |
| 2500.00 | 0.2435 | 0.2038 | 0.2237 | 0.2022 |
| 5000.00 | 0.6415 | 0.5878 | 0.6147 | 0.5932 |
| 10000.00 | 1.5460 | 1.4580 | 1.5020 | 1.4806 |
| 20000.00 | 2.9160 | 2.8280 | 2.8720 | 2.8506 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(pg/ml) | 369.9 | 2036.2 | 5695.3 | 384.4 | 2081.6 | 5790.9 |
| Standard Deviation | 34.0 | 104.1 | 314.4 | 45.2 | 113.9 | 357.2 |
| Coefficient of Variation(%) | 8.6 | 5.1 | 5.5 | 8.1 | 6.6 | 6.7 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human IL-13 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 92% to 109% with an overall mean recovery of 103%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
| Serum | 30 | n.d.-1456.69 | 43 | 447.82 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of IL-13 in this assay. No medical histories were available for the donors.
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