Human IL-17E ELISA Kit
Key features and details
- Specification:
- Sensitivity:
- Standard Curve Range:
- Standard Curve Gradient:
- Number of Incubations:
- Sample Volume:
- Assay type:
- Operation Duration:
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Brand:
CAT.NO. : AEH0066
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Product Details
Background
The IL-17 family is comprised of at least six proinflammatory cytokines that share a conserved cysteine-knot structure but diverge at the N-terminus. IL-17 family members are glycoproteins secreted as dimers that induce local cytokine production and recruit granulocytes to sites of inflammation. IL-17 is induced by IL-15 and IL-23, mainly in activated CD4+ T cells distinct from Th1 or Th2 cells. IL-17F is the most homologous to IL-17, but is induced only by IL-23 in activated monocytes. IL-17B binds the IL-17B receptor, but not the IL-17 receptor; it is most homologous with IL-17D, which is expressed by resting CD4+ T cells and CD19+ B cells. IL-17E is mainly produced by Th2 cells and recruits eosinophils to lung tissue. IL-17C has a very restricted expression pattern but has been detected in adult prostate and fetal kidney libraries.
Typical data
| pg/ml | O.D. | Average | Corrected | |
| 0.00 | 0.0111 | 0.0117 | 0.0114 | |
| 2.74 | 0.0171 | 0.0175 | 0.0173 | 0.0059 |
| 8.23 | 0.0323 | 0.0338 | 0.0331 | 0.0217 |
| 24.69 | 0.0691 | 0.0741 | 0.0716 | 0.0602 |
| 74.07 | 0.1946 | 0.2067 | 0.2007 | 0.1893 |
| 222.22 | 0.5879 | 0.5472 | 0.5676 | 0.5562 |
| 666.67 | 1.5650 | 1.5660 | 1.5655 | 1.5541 |
| 2000.00 | 3.6870 | 3.7130 | 3.7000 | 3.6886 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(pg/ml) | 40.3 | 180.8 | 566.4 | 38.9 | 186.5 | 589.6 |
| Standard Deviation | 2.0 | 10.2 | 57.1 | 2.3 | 8.8 | 35.4 |
| Coefficient of Variation(%) | 4.9 | 5.7 | 10.1 | 5.8 | 4.7 | 6.0 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human IL-17E into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 83% to 125% with an overall mean recovery of 108%.
The recovery ranged from 83% to 125% with an overall mean recovery of 108%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
| Serum | 30 | n.d.-3.56 | 62.5 | 2.01 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated in this assay. Donor medical histories were not available.
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