Human IL-18BP ELISA Kit

Protocol Booklet

Key features and details

Specification: 96 Test
Sensitivity: 1.67 pg/ml (50 μl)；1.70 pg/ml (10 μl)
Standard Curve Range: 13.72~10000 pg/ml
Standard Curve Gradient: 7 Points/3 Folds
Number of Incubations: 2
Sample Volume: 50 μl/10 μl
Assay type: Sandwich Elisa
Operation Duration: 120min

Brand:

CAT.NO. : AEH0131

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Size:

96T

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Product Details

Background

Interleukin-18-binding protein (IL18BP) is also known as Tadekinig-alfa, IL18BPa, which functions as an IL18 inhibitor. IL18BP is a secreted protein which contains one Ig-like C2-type (immunoglobulin-like) domain. IL18BP does not have a transmembrane domain and hence is not anchored to the cell membrane. IL18BP is strongly expressed in heart, lung, placenta and spleen. Isoform A of IL18BP binds to IL-18 and inhibits its activity. IL18BP also functions as an inhibitor of the early TH1 cytokine response. IL18BP suppresses the production of IFN-gamma resulting in reduced T-helper type 1 immune responses

typical data

pg/ml	O.D.		Average	Corrected
0.00	0.0229	0.0213	0.0221
13.72	0.0390	0.0375	0.0383	0.0162
41.15	0.0764	0.0680	0.0722	0.0501
123.46	0.1688	0.1584	0.1636	0.1415
370.37	0.4373	0.4087	0.4230	0.4009
1111.11	1.1060	1.0640	1.0850	1.0629
3333.33	2.4970	2.4830	2.4900	2.4679
10000.00	3.7010	3.7690	3.7350	3.7129

precision

	Intra-assay Precision			Inter-assay Precision
Sample Number	S1	S2	S3	S1	S2	S3
Sample Number	22	22	22	6	6	6
Average（pg/ml）	230.8	939.3	3046.5	213.2	861.2	2715.9
Standard deviation	8.9	64.3	188.3	14.1	48.6	71.8
Coefficient of variation（%）	3.8	6.8	6.2	6.6	5.6	2.6

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.

spike recovery

The spike recovery was evaluated by spiking 3 levels of human IL-18BP into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 87% to 106% with an overall mean recovery of 100%.

samplevalues

Sample Matrix

Sample

Evaluated

Range

(ng/ml)

Detectable

Mean of Detectable

(ng/ml)

Serum

22.78-56.27

100.0

32.77

Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of IL-18BP in this assay. No medical histories were available for the donors.

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