Human IL-21 ELISA Kit
Protocol Booklet
Key features and details
- Specification:
- Sensitivity:
- Standard Curve Range:
- Standard Curve Gradient:
- Number of Incubations:
- Sample Volume:
- Assay type:
- Operation Duration:
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Brand:
CAT.NO. : AEH0018
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Size:
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Product Details
Background
IL-21 (Interleukin-21) is member of the family of cytokines that utilize the common gamma chain as a receptor subunit. This subunit is also a component of the receptors for IL-2, IL-4, IL-7, IL-9, and IL-15. IL-21 is produced by activated T follicular helper cells (Tfh), Th17 cells, and NKT cells. It exerts its biological effects through a heterodimeric receptor complex of the common gamma chain and the IL-21-specific IL-21 R. IL-21 supports Tfh cell development, immunoglobulin affinity maturation, plasma cell differentiation, B cell memory responses, and the migration of dendritic cells to draining lymph nodes. It costimulates the activation, proliferation, and survival of CD8+ T cells and NKT cells and promotes Th17 cell polarization. It blocks the generation of regulatory T cells and their suppressive effects on CD4+ T cells. In addition, IL-21 suppresses cutaneous hypersensitivity reactions by limiting allergen-specific IgE production and mast cell degranulation.
Typical data
| pg/ml | O.D. | Average | Corrected | |
| 0.00 | 0.0142 | 0.0141 | 0.0142 | |
| 78.13 | 0.0189 | 0.0211 | 0.0200 | 0.0059 |
| 156.25 | 0.0347 | 0.0331 | 0.0339 | 0.0198 |
| 312.50 | 0.0671 | 0.0721 | 0.0696 | 0.0555 |
| 625.00 | 0.1874 | 0.1821 | 0.1848 | 0.1706 |
| 1250.00 | 0.5711 | 0.5513 | 0.5612 | 0.5471 |
| 2500.00 | 1.5113 | 1.4861 | 1.4987 | 1.4846 |
| 5000.00 | 3.3091 | 3.2221 | 3.2656 | 3.2515 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(pg/ml) | 86.4 | 416.3 | 1244.6 | 102.4 | 444.9 | 1444.7 |
| Standard Deviation | 7 | 24.1 | 68 | 9 | 15.2 | 107.9 |
| Coefficient of Variation(%) | 8.1 | 5.8 | 5.5 | 8.8 | 3.4 | 7.5 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human IL-21 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 93% to 124% with an overall mean recovery of 109%.
The recovery ranged from 93% to 124% with an overall mean recovery of 109%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
| Serum | 30 | n.d.-132.6 | 42 | 78.8 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of IL-21 in this assay. No medical histories were available for the donors.
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