Human IL-22 ELISA Kit
pg/ml |
O.D. |
Average |
Corrected |
|
0.00 |
0.0285 |
0.0284 |
0.0285 |
|
0.69 |
0.0412 |
0.0401 |
0.0407 |
0.0122 |
2.06 |
0.0647 |
0.0618 |
0.0633 |
0.0348 |
6.17 |
0.1402 |
0.1387 |
0.1395 |
0.1110 |
18.52 |
0.3698 |
0.3546 |
0.3622 |
0.3338 |
55.56 |
0.9875 |
0.9855 |
0.9865 |
0.9581 |
166.67 |
2.4529 |
2.3547 |
2.4038 |
2.3754 |
500.00 |
4.1289 |
4.1300 |
4.1295 |
4.1010 |
|
Intra-assay Precision |
|
Inter-assay Precision
|
||||
Sample Number
|
S1 |
S2 |
S3 |
S1 |
S2 |
S3 |
|
22 |
22 |
22 |
6 |
6 |
6 |
||
Average (pg/ml) |
10.7 |
48.9 |
172.6 |
10.6 |
48.6 |
176.8 |
|
Standard Deviation |
0.3 |
1.4 |
5.5 |
0.5 |
2.0 |
8.6 |
|
Coefficient of Variation (%) |
2.7 |
2.9 |
3.2 |
4.6 |
4.2 |
4.8 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
The spike recovery was evaluated by spiking 3 levels of human IL-22 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 91% to 117% with an overall mean recovery of 101%.
Sample Matrix |
Sample Evaluated |
Range (pg/ml) |
Detectable % |
Mean of Detectable (pg/ml) |
Serum |
30 |
6.48- 76.00 |
100 |
27.25 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of human IL-22 in this assay. No medical histories were available for the donors.
