Human IL-22 ELISA Kit

Key features and details

  • Specification: 96 Test
  • Sensitivity: 0.03 pg/ml (50 μl);0.21 pg/ml (10 μl)
  • Standard Curve Range: 0.69~500 pg/ml
  • Standard Curve Gradient: 7 Points/3 Folds
  • Number of Incubations: 2
  • Sample Volume: 50 μl/10 μl
  • Assay type: Sandwich Elisa
  • Operation Duration: 120min
  • Brand:
CAT.NO. : AEH0086
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Size:
96T
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Product Details
Background
Interleukin-22 (IL-22), also known as IL-10-related T cell-derived inducible factor (IL-TIF) was initially identified as a gene induced by IL-9 in mouse T cells and mast cells. Human IL-22 cDNA encodes a 179 amino acid (aa) residue protein with a putative 33 aa signal peptide that is cleaved to generate a 147 aa mature protein that shares approximately 79% and 22% aa sequence identity with mouse IL-22 and human IL-10, respectively. The human IL-22 gene is localized to chromosome 12q15. Although it exists as a single copy gene in human and in many mouse strains, the mouse IL-22 gene is duplicated in some mouse strains including C57B1/6, FVB and 129. The two mouse genes designated IL-TIF alpha and IL-TIF beta, share greater than 98% sequence homology in their coding region. IL-22 has been shown to activate STAT‑1 and STAT-3 in several hepatoma cell lines and upregulate the production of acute phase proteins. IL-22 is produced by normal T cells upon anti-CD3 stimulation in humans. Mouse IL-22 expression is also induced in various organs upon lipopolysaccharide injection, suggesting that IL-22 may be involved in inflammatory responses. The functional IL-22 receptor complex consists of two receptor subunits, IL-22R (previously an orphan receptor named CRF2-9) and IL-10R beta (previously known as CRF2-4), belonging to the class II cytokine receptor family.
Tipical Data

pg/ml

O.D.

Average

Corrected

0.00 

0.0285 

0.0284 

0.0285 

 

0.69 

0.0412 

0.0401 

0.0407 

0.0122 

2.06 

0.0647 

0.0618 

0.0633 

0.0348 

6.17 

0.1402 

0.1387 

0.1395 

0.1110 

18.52 

0.3698 

0.3546 

0.3622 

0.3338 

55.56 

0.9875 

0.9855 

0.9865 

0.9581 

166.67 

2.4529 

2.3547 

2.4038 

2.3754 

500.00 

4.1289 

4.1300 

4.1295 

4.1010 

Precision

 

 

Intra-assay Precision

 

 

 

 

Inter-assay Precision

 

 

Sample

Number

 

S1

S2

S3

S1

S2

S3

22

22

22

6

6

6

Average

(pg/ml)

10.7

48.9

172.6

10.6

48.6

176.8

Standard 

Deviation

0.3

1.4

5.5

0.5

2.0

8.6

Coefficient

 of

 Variation

(%)

2.7

2.9

3.2

4.6

4.2

4.8


Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.

Spike Recovery

The spike recovery was evaluated by spiking 3 levels of human IL-22 into health human serum sample. The un-spiked serum was used as blank in this experiment.

The recovery ranged from 91% to 117% with an overall mean recovery of 101%. 

Sample Values

Sample

Matrix

Sample 

Evaluated

Range

(pg/ml)

Detectable

%

Mean of Detectable

(pg/ml)

Serum

30

6.48-

76.00

100

27.25


Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of human IL-22 in this assay. No medical histories were available for the donors.

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