Human IL-31 ELISA Kit
Key features and details
- Specification:
- Sensitivity:
- Standard Curve Range:
- Standard Curve Gradient:
- Number of Incubations:
- Sample Volume:
- Assay type:
- Operation Duration:
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Brand:
Product Details
Human Interleukin-31 (IL-31) is a 24 kDa, short-chain member of the alpha -helical family of cytokines. The human IL-31 cDNA encodes a 164 amino acid (aa) precursor that contains a 23 aa signal peptide and a 141 aa mature protein. The mature region shows four alpha -helices which would be expected to show a typical up‑up‑down‑down topology. Human and mouse IL-31 share 24% aa sequence identity in the mature region. IL-31 is mainly associated with activated T cells and preferentially expressed by Th2 rather than Th1 cells. IL-31 signals via a heterodimeric receptor complex composed of a 120 kDa, gp130-related molecule termed IL‑31 RA (also GPL and GLM-R) and the 180 kDa oncostatin M receptor (OSM R beta ). In the complex, IL-31 directly binds to GPL, not OSM R. IL-31 signaling has been shown to involve the Jak/STAT pathway, the PI3 kinase/AKT cascade, and the MAP kinase pathway. Although multiple isoforms of IL-31 RA are known, only a form that contains the entire length of the cytoplasmic domain is signaling-capable. The IL-31 receptor is constitutively expressed by keratinocytes and up‑regulated by IFN-gamma on monocytes. Studies using transgenic mice indicate that IL-31 may contribute to the pruritis (itching) associated with nonatopic dermatitis.
|
pg/ml |
O.D. |
Average |
Corrected |
|
|
0.00 |
0.0165 |
0.0192 |
0.0179 |
|
|
1.37 |
0.0268 |
0.0277 |
0.0273 |
0.0094 |
|
4.12 |
0.0450 |
0.0391 |
0.0421 |
0.0242 |
|
12.35 |
0.0782 |
0.0770 |
0.0776 |
0.0598 |
|
37.04 |
0.2055 |
0.1949 |
0.2002 |
0.1824 |
|
111.11 |
0.5257 |
0.5334 |
0.5296 |
0.5117 |
|
333.33 |
1.4740 |
1.5710 |
1.5225 |
1.5047 |
|
1000.00 |
3.7040 |
3.7270 |
3.7155 |
3.6977 |
|
Intra-assay Precision |
Inter-assay Precision |
|||||
|
Sample Number |
S1 |
S2 |
S3 |
S1 |
S2 |
S3 |
|
22 |
22 |
22 |
6 |
6 |
6 |
|
|
Average(pg/ml) |
305.1 |
104.3 |
24.3 |
338.2 |
112.0 |
23.2 |
|
Standard Deviation |
12.7 |
4.2 |
1.3 |
18.8 |
2.8 |
1.5 |
|
Coefficient of Variation(%) |
4.2 |
4.0 |
5.2 |
5.6 |
2.5 |
6.5 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
The recovery ranged from 98% to 124% with an overall mean recovery of 114%.
|
Sample Matrix |
Sample Evaluated |
Range (pg/ml) |
Detectable (%) |
Mean of Detectable (pg/ml) |
|
Serum |
30 |
n.d.-2.34 |
69 |
1.16 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of IL-31 in this assay. No medical histories were available for the donors.
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