Human IL-3R alpha/CD123 ELISA Kit

Protocol Booklet

Key features and details

Specification: 96 Test
Sensitivity: 22.75 pg/ml (50 μl)；129.58 pg/ml (10 μl)
Standard Curve Range: 156.25~10000 pg/ml
Standard Curve Gradient: 7 Points
Number of Incubations: 2
Sample Volume: 50 μl/10 μl
Assay type: Sandwich Elisa
Operation Duration: 120min

Brand:

CAT.NO. : AEH0134

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Size:

96T

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Product Details

Background

Interleukin 3 receptor alpha (low affinity) (IL3RA), also known as CD123 (Cluster of Differentiation 123) is a 70-kD glycoprotein member of the hematopoietin receptor superfamily. This protein associates with a beta subunit common to the receptors for IL-5 and granulocyte-macrophage colony-stimulating factor (GM-CSF) to form a high-affinity receptor for IL-3. The interleukin-3 receptor α chain (CD123) has been identified as a potential immunotherapeutic target because it is overexpressed in AML compared with normal hematopoietic stem cells.

typical data

pg/ml	O.D.		Average	Corrected
0.00	0.0491	0.0475	0.0483
156.25	0.0991	0.0987	0.0989	0.0506
312.50	0.1488	0.1489	0.1489	0.1006
625.00	0.2471	0.2517	0.2494	0.2011
1250.00	0.4688	0.4276	0.4482	0.3999
2500.00	0.8168	0.8312	0.8240	0.7757
5000.00	1.5140	1.5180	1.5160	1.4677
10000.00	2.7410	2.7560	2.7485	2.7002

precision

	Intra-assay Precision			Inter-assay Precision
Sample Number	S1	S2	S3	S1	S2	S3
Sample Number	22	22	22	6	6	6
Average（pg/ml）	219.2	1152.6	3499.9	194.9	1053.1	3264.2
Standard deviation	12.1	67.3	170.5	12.8	58.3	145.9
Coefficient of variation（%）	5.5	5.8	4.9	6.5	5.5	4.5

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision

spike recovery

The spike recovery was evaluated by spiking 3 levels of human IL-3R alpha/CD123 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 80% to 95% with an overall mean recovery of 86%

sample values

Sample Matrix

Sample

Evaluated

Range

(pg/ml)

Detectable

Mean of Detectable

(pg/ml)

Serum

804.66-2639.86

100

1600.42

Serum/Plasma - Thirty samples from apparently healthy volunteers were evaluated for the presence of human IL-3R alpha/CD123 in this assay. No medical histories were available for the donors.

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