Human IL-4R ELISA Kit
Protocol Booklet
Key features and details
- Specification:
- Sensitivity:
- Standard Curve Range:
- Standard Curve Gradient:
- Number of Incubations:
- Sample Volume:
- Assay type:
- Operation Duration:
-
Brand:
CAT.NO. : AEH0145
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Product Details
Background
nterleukin 4 (IL-4) is a pleiotropic cytokine produced by activated T cells, mast cells, and basophils. The biological functions of IL-4 are mediated by the binding of IL‑4 to high-affinity cell surface receptor complexes. Two types of IL-4 receptor complexes have been described. The type I IL-4 receptor complex is composed of a high-affinity IL-4-binding subunit (referred to as IL-4 R alpha ) and the common gamma chain that does not bind IL-4 by itself. The type II IL-4 receptor complex is composed of IL-4 R alpha and IL-13 R alpha 1. Besides IL-4 signals, the type II IL-4 receptor complex can also transduce IL-13 signals. In the type II complex, the IL-4 R alpha subunit binds only IL-4 and not IL-13. Similarly, the IL-13 R alpha 1 subunit binds only IL-13 and not IL-4. The cDNA clones for both the human and mouse IL-4 R alpha have been isolated and shown to encode an approximately 140 kDa type I transmembrane protein with a large cytoplasmic domain that is essential for signal transduction. In mouse cells, an alternatively spliced variant encoding a soluble secreted IL-4 R alpha isoform has also been identified. Naturally occurring soluble IL-4 R alpha that binds IL-4 with high-affinity has been found in mouse and human biological fluids.
Typical data
| pg/ml | O.D. | Average | Corrected | |
| 0.00 | 0.0238 | 0.0229 | 0.0233 | |
| 2.74 | 0.0397 | 0.0423 | 0.0410 | 0.0177 |
| 8.23 | 0.0545 | 0.0561 | 0.0553 | 0.0320 |
| 24.69 | 0.1156 | 0.1163 | 0.1160 | 0.0926 |
| 74.07 | 0.2897 | 0.2685 | 0.2791 | 0.2558 |
| 222.22 | 0.7496 | 0.7079 | 0.7288 | 0.7054 |
| 666.67 | 1.8710 | 1.8480 | 1.8595 | 1.8362 |
| 2000.00 | 3.6970 | 3.7390 | 3.7180 | 3.6947 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(pg/ml) | 33.7 | 186.6 | 623.1 | 33.5 | 171.6 | 477.7 |
| Standard Deviation | 1.9 | 9.4 | 24.2 | 2.2 | 7.1 | 8.3 |
| Coefficient of Variation(%) | 5.5 | 5.0 | 3.9 | 6.4 | 4.1 | 1.7 |
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human IL-4R into health human serum sample. The un-spiked serum was used as blank in this experiment. The recovery ranged from 80% to 130% with an overall mean recovery of 113%.
Sample Values
|
Sample Matrix |
Sample Evaluated |
Range (pg/ml) |
Detectable (%) |
Mean of Detectable (pg/ml) |
|
Serum |
30 |
122-235.96 |
100 |
176.01 |
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