Human IL27 ELISA Kit
Key features and details
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- 应用:
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- 靶点名称:
- 偶联标记:
- 偶联标记说明:
- 灵敏度:
- 样品类型:
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Brand:
CAT.NO. : ARG80119
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Product Details
内含物
| 货号 | 内含物名称 | 包装 | 温度 |
|---|---|---|---|
| ARG80119-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG80119-002 | Standard (Lyophilized) | 2 X 4 ng/vial | 4°C |
| ARG80119-003 | Standard diluent buffer | 20 ml | 4°C |
| ARG80119-004 | Antibody conjugate concentrate | 400 µl | 4°C |
| ARG80119-005 | Antibody diluent buffer | 16 ml | 4°C |
| ARG80119-006 | HRP-Streptavidin concentrate | 400 µl | 4°C (Protect from light) |
| ARG80119-007 | HRP-Streptavidin diluent buffer | 16 ml | 4°C |
| ARG80119-008 | 20X Wash buffer | 50 ml | 4°C |
| ARG80119-009 | TMB substrate | 12ml | 4°C (Protect from light) |
| ARG80119-010 | STOP solution | 12ml | 4°C |
| ARG80119-011 | Plate sealer | 4 strips | Room temperature |
概述
| 产品描述 | ARG80119 Human IL27 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human IL27 in serum, plasma and cell culture supernatants. |
|---|---|
| 反应物种 | Hu |
| 应用 | ELISA |
| 特异性 | No significant cross-reactivity or interference with Human IL-12 p70, IL-12/IL-23 p40；mouse IL-27 |
| 靶点名称 | IL27 |
| 偶联标记 | HRP |
| 偶联标记说明 | Substrate: TMB and read at 450 nm |
| 灵敏度 | 23.44 pg/ml |
| 样品类型 | Serum, plasma and cell culture supernatants |
| 标准范围 | 46.875 - 3000 pg/ml |
| 样本量 | 100 µl |
| 精确 | CV: less than10% |
| 別名 | IL27; Interleukin 27; IL27A; P28; IL27p28; IL-27A; IL-27; IL30; Interleukin-27 Subunit Alpha; IL-27 Subunit Alpha; Interleukin-30; MGC71873; IL-27-A; IL27-A; IL-27 P28 Subunit; Interleukin 30 |
应用说明
| 检测时间 | 4 hours |
|---|
属性
| 形式 | 96 well |
|---|---|
| 存放说明 | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | IL27 |
| 全名 | interleukin 27 |
| 背景介绍 | The protein encoded by this gene is one of the subunits of a heterodimeric cytokine complex. This protein is related to interleukin 12A (IL12A). It interacts with Epstein-Barr virus induced gene 3 (EBI3), a protein similar to interleukin 12B (IL12B), and forms a complex that has been shown to drive rapid expansion of naive but not memory CD4(+) T cells. The complex is also found to synergize strongly with interleukin 12 to trigger interferon gamma (IFNG) production of naive CD4(+) T cells. The biological effects of this cytokine are mediated by the class I cytokine receptor (WSX1/TCRR). [provided by RefSeq, Jul 2008] |
| 生物功能 | Associates with EBI3 to form the IL-27 interleukin, a heterodimeric cytokine which functions in innate immunity. IL-27 has pro- and anti-inflammatory properties, that can regulate T-helper cell development, suppress T-cell proliferation, stimulate cytotoxic T-cell activity, induce isotype switching in B-cells, and that has diverse effects on innate immune cells. Among its target cells are CD4 T-helper cells which can differentiate in type 1 effector cells (TH1), type 2 effector cells (TH2) and IL17 producing helper T-cells (TH17). It drives rapid clonal expansion of naive but not memory CD4 T-cells. It also strongly synergizes with IL-12 to trigger interferon-gamma/IFN-gamma production of naive CD4 T-cells, binds to the cytokine receptor WSX-1/TCCR which appears to be required but not sufficient for IL-27-mediated signal transduction. IL-27 potentiate the early phase of TH1 response and suppress TH2 and TH17 differentiation. It induces the differentiation of TH1 cells via two distinct pathways, p38 MAPK/TBX21- and ICAM1/ITGAL/ERK-dependent pathways. It also induces STAT1, STAT3, STAT4 and STAT5 phosphorylation and activates TBX21/T-Bet via STAT1 with resulting IL12RB2 up-regulation, an event crucial to TH1 cell commitment. It suppresses the expression of GATA3, the inhibitor TH1 cells development. In CD8 T-cells, it activates STATs as well as GZMB. IL-27 reveals to be a potent inhibitor of TH17 cell development and of IL-17 production. Indeed IL27 alone is also able to inhibit the production of IL17 by CD4 and CD8 T-cells. While IL-27 suppressed the development of proinflammatory Th17 cells via STAT1, it inhibits the development of anti-inflammatory inducible regulatory T-cells, iTreg, independently of STAT1. IL-27 has also an effect on cytokine production, it suppresses proinflammatory cytokine production such as IL2, IL4, IL5 and IL6 and activates suppressors of cytokine signaling such as SOCS1 and SOCS3. Apart from suppression of cytokine production, IL-27 also antagonizes the effects of some cytokines such as IL6 through direct effects on T-cells. Another important role of IL-27 is its antitumor activity as well as its antiangiogenic activity with activation of production of antiangiogenic chemokines such as IP-10/CXCL10 and MIG/CXCL9. In vein endothelial cells, it induces IRF1/interferon regulatory factor 1 and increase the expression of MHC class II transactivator/CIITA with resulting up-regulation of major histocompatibility complex class II. IL-27 also demonstrates antiviral activity with inhibitory properties on HIV-1 replication. [UniProt] |
| 产品亮点 | Related products: IL27 antibodies; IL27 ELISA Kits; IL27 recombinant proteins; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| 研究领域 | Cancer kit; Immune System kit |
| 翻译后修饰 | O-glycosylated. |
检测图片 (1)
ARG80119 Human IL27 ELISA Kit standard curve image
ARG80119 Human IL27 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
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