Human Intact PTH / Parathyroid Hormone ELISA Kit
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- 偶联标记:
- 偶联标记说明:
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CAT.NO. : ARG80779
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Product Details
概述
| 产品描述 | ARG80779 Human Intact PTH / Parathyroid Hormone ELISA Kit is intended for the quantification of Intact-PTH (Parathyroid Hormone) in human serum and plasma (EDTA). |
|---|---|
| 反应物种 | Hu |
| 应用 | ELISA |
| 靶点名称 | Intact PTH / Parathyroid Hormone |
| 偶联标记 | HRP |
| 偶联标记说明 | Substrate: TMB and read at 450 nm or 405 nm |
| 灵敏度 | 1.57 pg/ml |
| 样品类型 | Serum and plasma (EDTA). |
| 标准范围 | 11.1 - 971 pg/ml |
| 样本量 | 25 µl |
| 別名 | PTH1; Parathyroid hormone; Parathyrin; Parathormone; PTH |
应用说明
| 检测时间 | 3 h, 30 min (RT/shaker) |
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属性
| 形式 | 96 well |
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| 存放说明 | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | PTH |
| 全名 | parathyroid hormone |
| 背景介绍 | PTH (Parathyroid hormone, Parathormone, Parathyrin) is biosynthesized in the parathyroid gland as a pre-proparathyroid hormone, a larger molecular precursor consisting of 115 amino acids. Following sequential intracellular cleavage of a 25-amino acid sequence, preproparathyroid hormone is converted to an intermediate, a 90-amino acid polypetide, proparathyroid hormone. With additional proteolytic modification, proparathyroid hormone is then converted to parathyroid hormone, an 84 amino acid polypeptide. In healthy individuals, regulation of parathyroid hormone secretion normally occurs via a negative feedback action of serum calcium on the parathyroid glands. Intact PTH is biologically active and clears very rapidly from the circulation with a half-life of less than four minutes. PTH undergoes proteolysis in the parathyroid glands, but mostly peripherally, particularly in the liver but also in the kidneys and bone, to give N-terminal fragments and longer lived C-terminal and midregion fragments. In subjects with renal insufficiency, C‑terminal and midregion PTH assays typically give elevated PTH results, as reflected by impaired renal clearance. Intact PTH assays are important for the differentiation of primary hyperparathyroidism from other (non-parathyroid-mediated) forms of hypercalcemia, such as malignancy, sarcoidosis and thyrotoxicosis. The measurement of parathyroid hormone is the most specific way of making the diagnosis of primary hyperparathyroidism. In the presence of hypercalcemia, an elevated level of parathyroid hormone virtually establishes the diagnosis. In over 90% of patients with primary hyperparathyroidism, the parathyroid hormone will be elevated. The most common other cause of hypercalcemia, namely hypercalcemia of malignancy, is associated with suppressed levels of parathyroid hormone or PTH levels within the normal range. When intact PTH level is plotted against serum calcium, the intact PTH concentration for patients with hypercalcemia of malignancy is almost always found to be inappropriately low when interpreted in view of the elevated serum calcium. Unlike C-terminal and midregion PTH, which typically are grossly elevated in subjects with renal insufficiency, intact PTH assays are less influenced by the declining renal function. PTH values are typically undetectable in hypocalcemia due to total hypoparathyroidism, but are found within the normal range in hypocalcemia due to partial loss or inhibition of parathyroid function. |
| 产品亮点 | Related products: PTH antibodies; PTH ELISA Kits; PTH Duos / Panels; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| 研究领域 | Signaling Transduction kit |
检测图片 (2)
ARG80779 Human Intact PTH / Parathyroid Hormone ELISA Kit example of standard curve image
ARG80779 Human Intact PTH / Parathyroid Hormone ELISA Kit results of a typical standard run with optical density reading at 450 nm.
ARG80779 Human Intact PTH / Parathyroid Hormone ELISA Kit example of standard curve image
ARG80779 Human Intact PTH / Parathyroid Hormone ELISA Kit results of a typical standard run with optical density reading at 405 nm.
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