Human Irisin ELISA Kit
Key features and details
- Specification:
- Sensitivity:
- Standard Curve Range:
- Standard Curve Gradient:
- Number of Incubations:
- Sample Volume:
- Assay type:
- Operation Duration:
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Brand:
CAT.NO. : AEH0081
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Product Details
Background
Irisin (also known as FNDC5) is a 12 kDa glycosylated polypeptide hormone that regulates energy metabolism, stem cell differentiation, and neuronal development. Human Irisin is synthesized as a 212 amino acid (aa) precursor encoding a type 1 transmembrane protein with a 121 aa extracellular domain (ECD), a 21 aa transmembrane domain, and a 39 aa cytoplasmic domain. The ECD of Irisin contains a fibronectin type III domain and multiple glycosylation sites. The ECD is proteolytically cleaved to release the 112 aa soluble Irisin hormone into circulation. Mature human, mouse, and rat Irisin share 100% sequence identity. Expression of Irisin is induced in skeletal muscle and subcutaneous adipose tissue during and shortly after exercise. Irisin induces expression of peroxisome proliferator-activated receptor gamma co-activator 1 alpha (PGC1 alpha ) and uncoupling protein-1 (UCP1), mitochondrial-associated metabolic proteins. Irisin induces the transition of white adipose tissue into more metabolically active beige adipose tissue. In mice, expression of Irisin has been shown to regulate obesity and diabetes. A similar function in humans is suggested. Irisin also regulates neuronal cell differentiation and neurite outgrowth in the brain and is involved in the differentiation of osteoblasts.
Typical data
| pg/ml | O.D. | Average | Corrected | |
| 0.00 | 0.0272 | 0.0286 | 0.0279 | |
| 15.63 | 0.0627 | 0.0626 | 0.0627 | 0.0348 |
| 31.25 | 0.1025 | 0.1015 | 0.1020 | 0.0741 |
| 62.50 | 0.2029 | 0.2004 | 0.2017 | 0.1738 |
| 125.00 | 0.4505 | 0.4487 | 0.4496 | 0.4217 |
| 250.00 | 1.1510 | 1.1050 | 1.1280 | 1.1001 |
| 500.00 | 2.6620 | 2.5750 | 2.6185 | 2.5906 |
| 1000.00 | 4.1606 | 4.1981 | 4.1794 | 4.1515 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(pg/ml) | 21.5 | 97.1 | 301 | 19.5 | 94.8 | 305.4 |
| Standard Deviation | 0.6 | 0.9 | 6.7 | 1.2 | 1.6 | 6.3 |
| Coefficient of Variation(%) | 2.7 | 1 | 2.2 | 5.9 | 1.7 | 2.1 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human Irisin into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 86% to 118% with an overall mean recovery of 98%.
The recovery ranged from 86% to 118% with an overall mean recovery of 98%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
| Serum | 30 | 11.14-100.57 | 100 | 26.84 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of VEGF in this assay. No medical histories were available for the donors.
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