Human Leptin ELISA kit
Key features and details
- 反应物种:
- 应用:
- 靶点名称:
- 偶联标记:
- 灵敏度:
- 样品类型:
- 标准范围:
- 样本量:
-
Brand:
CAT.NO. : ARG83757
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Product Details
概述
| 产品描述 | ARG83757 Human Leptin ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human Leptin in serum, plasma and cell culture supernatants. |
|---|---|
| 反应物种 | Hu |
| 应用 | ELISA |
| 靶点名称 | Leptin |
| 偶联标记 | HRP |
| 偶联标记说明 | Substrate: TMB and read at 450 nm. |
| 灵敏度 | 31.25 pg/ml |
| 样品类型 | Serum, plasma and cell culture supernatants. |
| 标准范围 | 62.5 - 4000 pg/ml |
| 样本量 | 50 µl |
| 精确 | Intra-Assay CV: <10% Inter-Assay CV: <10% |
| 別名 | LEP; Leptin; OBS; OB; Leptin (Murine Obesity Homolog); Leptin (Obesity Homolog, Mouse); Obesity Factor; Obese Protein; Obese, Mouse, Homolog Of; LEPD |
应用说明
| 检测时间 | 4 hours |
|---|
属性
| 形式 | 96 well |
|---|---|
| 存放说明 | Store the kit at 4°C, -20°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | LEP |
| 全名 | Leptin |
| 背景介绍 | This gene encodes a protein that is secreted by white adipocytes into the circulation and plays a major role in the regulation of energy homeostasis. Circulating leptin binds to the leptin receptor in the brain, which activates downstream signaling pathways that inhibit feeding and promote energy expenditure. This protein also has several endocrine functions, and is involved in the regulation of immune and inflammatory responses, hematopoiesis, angiogenesis, reproduction, bone formation and wound healing. Mutations in this gene and its regulatory regions cause severe obesity and morbid obesity with hypogonadism in human patients. A mutation in this gene has also been linked to type 2 diabetes mellitus development. [provided by RefSeq, Aug 2017] |
| 生物功能 | Increases CD4+CD25- T-cell proliferation and reduces autophagy during TCR (T-cell receptor) stimulation, through MTOR signaling pathway activation and BCL2 up-regulation. [Uniprot] |
检测图片
ARG83757 Human Leptin ELISA Kit standard curve image
ARG83757 Human Leptin ELISA Kit results of a typical standard run with optical density reading at 450 nm.
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