Human Lipocalin-2/NGAL ELISA Kit
Key features and details
- Specification:
- Sensitivity:
- Standard Curve Range:
- Standard Curve Gradient:
- Number of Incubations:
- Sample Volume:
- Assay type:
- Operation Duration:
-
Brand:
CAT.NO. : AEHY0166
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Size:
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Product Details
Background
The Lipocalin family comprises a diverse group of mostly secreted soluble proteins that bind hydrophobic ligands and act as transporters, carrying small molecules to specific cells. Lipocalins are related by possessing an 8-stranded beta-barrel structure. Lipocalin-1, also named tear lipocalin (TL), von Ebners gland protein (VEG) and tear pre-albumin, binds a large number of hydrophobic molecules and exhibits cysteine proteinase inhibitor and endonuclear activities. Lipocalin-2, also known as neutrophil gelatinase-associated lipocalin (NGAL), is a component of granules in neutrophils from tissues that are normally exposed to microorganisms and is upregulated during inflammation. Lipocalin-2 can form homodimers and can heterodimerize with the neutrophil gelatinase MMP-9.
Typical data
|
ng/ml |
O.D. |
Average |
Corrected |
|
|
0.00 |
0.0126 |
0.0115 |
0.0121 |
|
|
0.14 |
0.0241 |
0.0241 |
0.0241 |
0.0121 |
|
0.41 |
0.0450 |
0.0408 |
0.0429 |
0.0309 |
|
1.23 |
0.0977 |
0.0952 |
0.0965 |
0.0844 |
|
3.70 |
0.2584 |
0.2660 |
0.2622 |
0.2502 |
|
11.11 |
0.7241 |
0.7346 |
0.7294 |
0.7173 |
|
33.33 |
1.8220 |
1.8880 |
1.8550 |
1.8430 |
|
100.00 |
3.2360 |
3.2210 |
3.2285 |
3.2165 |
Precision
|
Intra-assay Precision |
Inter-assay Precision |
|||||
|
Sample Number |
S1 |
S2 |
S3 |
S1 |
S2 |
S3 |
|
22 |
22 |
22 |
6 |
6 |
6 |
|
|
Average(ng/ml) |
2.3 |
12.0 |
41.8 |
1.7 |
9.7 |
38.1 |
|
Standard Deviation |
0.1 |
0.3 |
1.8 |
0.1 |
0.5 |
1.3 |
|
Coefficient of Variation(%) |
4.7 |
2.7 |
4.3 |
4.5 |
4.8 |
3.4 |
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human Lipocalin-2/NGAL into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 92% to 105% with an overall mean recovery of 97%.
The recovery ranged from 92% to 105% with an overall mean recovery of 97%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (μg/ml) | Detectable (%) | Mean of Detectable (μg/ml) |
|---|---|---|---|---|
| Serum | 30 | 11.47-84.79 | 100 | 49.12 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of Lipocalin-2/NGAL in this assay. No medical histories were available for the donors.
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