Human MAdCAM1 ELISA Kit
Key features and details
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CAT.NO. : ARG82705
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Product Details
内含物
| 货号 | 内含物名称 | 包装 | 温度 |
|---|---|---|---|
| ARG82705-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG82705-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG82705-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG82705-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82705-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82705-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82705-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82705-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG82705-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG82705-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG82705-011 | Plate sealer | 4 strips | Room temperature |
概述
| 产品描述 | ARG82705 Human MAdCAM1 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human MAdCAM1 in serum, plasma (EDTA, heparin) and cell culture supernatants. |
|---|---|
| 反应物种 | Hu |
| 应用 | ELISA |
| 靶点名称 | MAdCAM1 |
| 偶联标记 | HRP |
| 偶联标记说明 | Substrate: TMB and read at 450 nm. |
| 灵敏度 | 16 pg/ml |
| 样品类型 | Serum, plasma (EDTA, heparin) and cell culture supernatants. |
| 标准范围 | 31.2 - 2000 pg/ml |
| 样本量 | 100 µl |
| 精确 | Intra-Assay CV: 6.5% Inter-Assay CV: 7.0% |
| 別名 | Mucosal addressin cell adhesion molecule 1; hMAdCAM-1; MAdCAM-1; MACAM1 |
应用说明
| 检测时间 | ~ 5 hours |
|---|
属性
| 形式 | 96 well |
|---|---|
| 存放说明 | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | Swiss-port # Q13477 Human Mucosal addressin cell adhesion molecule 1 |
|---|---|
| 基因名称 | MADCAM1 |
| 全名 | mucosal vascular addressin cell adhesion molecule 1 |
| 背景介绍 | The protein encoded by this gene is an endothelial cell adhesion molecule that interacts preferentially with the leukocyte beta7 integrin LPAM-1 (alpha4beta7), L-selectin, and VLA-4 (alpha4beta1) on myeloid cells to direct leukocytes into mucosal and inflamed tissues. It is a member of the immunoglobulin family and is similar to ICAM1 and VCAM1. At least seven alternatively spliced transcripts encoding different protein isoforms have been found for this gene, but the full-length nature of some variants has not been determined. [provided by RefSeq, Jul 2008] |
| 生物功能 | Cell adhesion leukocyte receptor expressed by mucosal venules, helps to direct lymphocyte traffic into mucosal tissues including the Peyer patches and the intestinal lamina propria. It can bind both integrin alpha-4/beta-7 and L-selectin, regulating both the passage and retention of leukocytes. Isoform 2, lacking the mucin-like domain, may be specialized in supporting integrin alpha-4/beta-7-dependent adhesion strengthening, independent of L-selectin binding. [UniProt] |
| 细胞定位 | Membrane; Single-pass type I membrane protein. [UniProt] |
| 产品亮点 | Related products: MAdCAM1 antibodies; MAdCAM1 ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| 翻译后修饰 | The Ser/Thr-rich mucin-like domain may provide possible sites for O-glycosylation. [UniProt] |
检测图片 (1)
ARG82705 Human MAdCAM1 ELISA Kit standard curve image
ARG82705 Human MAdCAM1 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
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