Human MERTK ELISA Kit
Key features and details
- Specification:
- Sensitivity:
- Standard Curve Range:
- Standard Curve Gradient:
- Number of Incubations:
- Sample Volume:
- Assay type:
- Operation Duration:
-
Brand:
CAT.NO. : AEHY0189
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Size:
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Product Details
Background
Tyrosine-protein kinase Mer(MERTK) is a member of the TYRO3/AXL/MER (TAM) receptor kinase family and encodes a transmembrane protein with two fibronectin type-III domains, two Ig-like C2-type (immunoglobulin-like) domains, and one tyrosine kinase domain. Following activation by ligand, interacts with GRB2 or PLCG2 and induces phosphorylation of MAPK1, MAPK2, FAK/PTK2 or RAC1. MERTK signaling plays a role in various processes such as macrophage clearance of apoptotic cells, platelet aggregation, cytoskeleton reorganization and engulfment. Functions in the retinal pigment epithelium (RPE) as a regulator of rod outer segments fragments phagocytosis. Plays also an important role in inhibition of Toll-like receptors (TLRs)-mediated innate immune response by activating STAT1, which selectively induces production of suppressors of cytokine signaling SOCS1 and SOCS3.
Typical data
|
ng/ml |
O.D. |
Average |
Corrected |
|
|
0.00 |
0.0114 |
0.0112 |
0.0113 |
|
|
0.27 |
0.0191 |
0.0184 |
0.0188 |
0.0075 |
|
0.82 |
0.0343 |
0.0338 |
0.0341 |
0.0228 |
|
2.47 |
0.0751 |
0.0732 |
0.0742 |
0.0629 |
|
7.41 |
0.1987 |
0.2044 |
0.2016 |
0.1903 |
|
22.22 |
0.4883 |
0.5378 |
0.5131 |
0.5018 |
|
66.67 |
1.4530 |
1.5690 |
1.5110 |
1.4997 |
|
200.00 |
3.4070 |
3.4100 |
3.4085 |
3.3972 |
Precision
|
Intra-assay Precision |
Inter-assay Precision |
|||||
|
Sample Number |
S1 |
S2 |
S3 |
S1 |
S2 |
S3 |
|
22 |
22 |
22 |
6 |
6 |
6 |
|
|
Average(ng/ml) |
3.7 |
21.9 |
76.7 |
3.0 |
17.4 |
58.8 |
|
Standard Deviation |
0.2 |
0.9 |
4.1 |
0.2 |
1.1 |
4.5 |
|
Coefficient of Variation(%) |
5.5 |
4.0 |
5.3 |
5.6 |
6.1 |
7.7 |
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human MERTK into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 70% to 105% with an overall mean recovery of 94%.
The recovery ranged from 70% to 105% with an overall mean recovery of 94%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (ng/ml) | Detectable (%) | Mean of Detectable (ng/ml) |
|---|---|---|---|---|
| Serum | 30 | 37.02-124.26 | 100 | 56.4 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of MERTK in this assay. No medical histories were available for the donors.
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