Human MERTK ELISA Kit
Key features and details
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- 反应物种:
- 应用:
- 特异性:
- 靶点名称:
- 偶联标记:
- 偶联标记说明:
- 灵敏度:
- 样品类型:
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Brand:
CAT.NO. : ARG81749
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Product Details
内含物
| 货号 | 内含物名称 | 包装 | 温度 |
|---|---|---|---|
| ARG81749-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81749-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81749-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81749-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81749-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81749-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81749-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81749-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81749-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81749-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81749-011 | Plate sealer | 4 strips | Room temperature |
概述
| 产品描述 | ARG81749 Human MERTK ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human MERTK in serum and cell culture supernatants. |
|---|---|
| 反应物种 | Hu |
| 应用 | ELISA |
| 特异性 | There is no detectable cross-reactivity with other relevant proteins. |
| 靶点名称 | MERTK |
| 偶联标记 | HRP |
| 偶联标记说明 | Substrate: TMB and read at 450 nm. |
| 灵敏度 | 78 pg/ml |
| 样品类型 | Serum and cell culture supernatants. |
| 标准范围 | 156 - 10000 pg/ml |
| 样本量 | 100 µl |
| 精确 | Intra-Assay CV: 5.0%; Inter-Assay CV: 5.7% |
| 別名 | c-mer; Receptor tyrosine kinase MerTK; c-Eyk; Tyro12; MER; RP38; EC 2.7.10.1; Proto-oncogene c-Mer; Tyrosine-protein kinase Mer |
应用说明
| 检测时间 | ~ 5 hours |
|---|
属性
| 形式 | 96 well |
|---|---|
| 存放说明 | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | MERTK |
| 全名 | MER proto-oncogene, tyrosine kinase |
| 背景介绍 | This gene is a member of the MER/AXL/TYRO3 receptor kinase family and encodes a transmembrane protein with two fibronectin type-III domains, two Ig-like C2-type (immunoglobulin-like) domains, and one tyrosine kinase domain. Mutations in this gene have been associated with disruption of the retinal pigment epithelium (RPE) phagocytosis pathway and onset of autosomal recessive retinitis pigmentosa (RP). [provided by RefSeq, Jul 2008] |
| 生物功能 | Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to several ligands including LGALS3, TUB, TULP1 or GAS6. Regulates many physiological processes including cell survival, migration, differentiation, and phagocytosis of apoptotic cells (efferocytosis). Ligand binding at the cell surface induces autophosphorylation of MERTK on its intracellular domain that provides docking sites for downstream signaling molecules. Following activation by ligand, interacts with GRB2 or PLCG2 and induces phosphorylation of MAPK1, MAPK2, FAK/PTK2 or RAC1. MERTK signaling plays a role in various processes such as macrophage clearance of apoptotic cells, platelet aggregation, cytoskeleton reorganization and engulfment. Functions in the retinal pigment epithelium (RPE) as a regulator of rod outer segments fragments phagocytosis. Plays also an important role in inhibition of Toll-like receptors (TLRs)-mediated innate immune response by activating STAT1, which selectively induces production of suppressors of cytokine signaling SOCS1 and SOCS3. [UniProt] |
| 产品亮点 | Related products: MERTK antibodies; MERTK ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| 翻译后修饰 | Autophosphorylated on Tyr-749, Tyr-753 and Tyr-754 in the activation loop allowing full activity. Autophosphorylated on Tyr-872 leading to recruitment of downstream partners of the signaling cascade such as PLCG2 (By similarity). [UniProt] |
检测图片 (1)
ARG81749 Human MERTK ELISA Kit standard curve image
ARG81749 Human MERTK ELISA Kit results of a typical standard run with optical density reading at 450 nm.
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