Human Mesothelin ELISA Kit

Key features and details

  • 产品描述: ARG81556 Human Mesothelin ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human Mesothelin in serum, plasma (heparin, EDTA), saliva, urine and cell culture supernatants.
  • 反应物种: Hu
  • 应用: ELISA
  • 特异性: There is no detectable cross-reactivity with other relevant proteins.
  • 靶点名称: Mesothelin
  • 偶联标记: HRP
  • 偶联标记说明: Substrate: TMB and read at 450 nm.
  • 灵敏度: 78 pg/ml
  • 样品类型: Serum, plasma (heparin, EDTA), saliva, urine and cell culture supernatants.
  • Brand:
CAT.NO. : ARG81556
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Product Details
内含物
货号内含物名称包装温度
ARG81556-001Antibody-coated microplate8 X 12 strips 4°C. Unused strips should be sealed tightly in the air-tight pouch.
ARG81556-002Standard2 X 10 ng/vial 4°C
ARG81556-003Standard/Sample diluent30 ml (Ready to use) 4°C
ARG81556-004Antibody conjugate concentrate (100X)1 vial (100 µl) 4°C
ARG81556-005Antibody diluent buffer12 ml (Ready to use) 4°C
ARG81556-006HRP-Streptavidin concentrate (100X)1 vial (100 µl) 4°C
ARG81556-007HRP-Streptavidin diluent buffer12 ml (Ready to use) 4°C
ARG81556-00825X Wash buffer20 ml 4°C
ARG81556-009TMB substrate10 ml (Ready to use) 4°C (Protect from light)
ARG81556-010STOP solution10 ml (Ready to use) 4°C
ARG81556-011Plate sealer4 strips Room temperature
概述
产品描述ARG81556 Human Mesothelin ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human Mesothelin in serum, plasma (heparin, EDTA), saliva, urine and cell culture supernatants.
反应物种Hu
应用ELISA
特异性There is no detectable cross-reactivity with other relevant proteins.
靶点名称Mesothelin
偶联标记HRP
偶联标记说明Substrate: TMB and read at 450 nm.
灵敏度78 pg/ml
样品类型Serum, plasma (heparin, EDTA), saliva, urine and cell culture supernatants.
标准范围156 - 10000 pg/ml
样本量100 µl
精确Intra-Assay CV: 5.4%
Inter-Assay CV: 6.6%
別名MPF; Mesothelin; CAK1 antigen; Pre-pro-megakaryocyte-potentiating factor; SMRP
应用说明
检测时间~ 5 hours
属性
形式96 well
存放说明Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components.
注意事项For laboratory research only, not for drug, diagnostic or other use.
生物信息
数据库连接

GeneID: 10232 Human MSLN

Swiss-port # Q13421 Human Mesothelin

基因名称MSLN
全名mesothelin
背景介绍This gene encodes a precursor protein that is cleaved into two products, megakaryocyte potentiating factor and mesothelin. Megakaryocyte potentiation factor functions as a cytokine that can stimulate colony formation in bone marrow megakaryocytes. Mesothelian is a glycosylphosphatidylinositol-anchored cell-surface protein that may function as a cell adhesion protein. This protein is overexpressed in epithelial mesotheliomas, ovarian cancers and in specific squamous cell carcinomas. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Apr 2010]
生物功能Membrane-anchored forms may play a role in cellular adhesion.

Megakaryocyte-potentiating factor (MPF) potentiates megakaryocyte colony formation in vitro. [UniProt]
产品亮点Related products:
Mesothelin antibodies; Mesothelin ELISA Kits;
New ELISA data calculation tool:
Simplify the ELISA analysis by GainData
翻译后修饰Both MPF and the cleaved form of mesothelin are N-glycosylated.

Proteolytically cleaved by a furin-like convertase to generate megakaryocyte-potentiating factor (MPF), and the cleaved form of mesothelin. [UniProt]
检测图片 (1)
  • ARG81556 Human Mesothelin ELISA Kit standard curve image

    ARG81556 Human Mesothelin ELISA Kit results of a typical standard run with optical density reading at 450 nm.

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