Human MICA ELISA Kit
Protocol Booklet
Key features and details
- Specification:
- Sensitivity:
- Standard Curve Range:
- Standard Curve Gradient:
- Number of Incubations:
- Sample Volume:
- Assay type:
- Operation Duration:
-
Brand:
CAT.NO. : AEH0044
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Size:
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Product Details
Background
MHC class I chain-related genes MICA and MICB are transmembrane glycoproteins that function as ligands for human NKG2D. The two proteins are highly related, sharing 85% amino acid identity, but are also polymorphic. Recognition of MICA or MICB by NKG2D results in the activation of NK cell cytolytic activity and/or cytokine production. MICA/B are minimally expressed on normal cells, but are frequently expressed on or shed from epithelial tumors and can be induced by bacterial and viral infections. MICA and MICB recognition is involved in tumor surveillance, viral infections, and autoimmune diseases.
Typical data
| pg/ml | O.D. | Average | Corrected | |
| 0.00 | 0.0202 | 0.0214 | 0.0208 | |
| 156.25 | 0.0808 | 0.0828 | 0.0818 | 0.0610 |
| 312.50 | 0.1430 | 0.1440 | 0.1435 | 0.1227 |
| 625.00 | 0.2682 | 0.2661 | 0.2672 | 0.2464 |
| 1250.00 | 0.5125 | 0.5294 | 0.5210 | 0.5002 |
| 2500.00 | 1.0160 | 1.0370 | 1.0265 | 1.0057 |
| 5000.00 | 1.9670 | 1.9410 | 1.9540 | 1.9332 |
| 10000.00 | 3.2950 | 3.4180 | 3.3565 | 3.3357 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(pg/ml) | 389.9 | 1231.6 | 3586.9 | 373.1 | 85.4 | 3682.9 |
| Standard Deviation | 21.2 | 45.6 | 111.9 | 4.6 | 41.5 | 85.4 |
| Coefficient of Variation(%) | 5.4 | 3.7 | 3.1 | 2.5 | 3.1 | 2.3 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human MICA into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 94% to 112% with an overall mean recovery of 106%.
The recovery ranged from 94% to 112% with an overall mean recovery of 106%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
| Serum | 30 | 469.25-3717.25 | 100 | 1351.05 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of IL-12/IL-23 p40 in this assay. No medical histories were available for the donors.
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