Human MICA ELISA Kit
Key features and details
- 产品描述:
- 反应物种:
- 应用:
- 靶点名称:
- 偶联标记:
- 偶联标记说明:
- 灵敏度:
- 样品类型:
- 标准范围:
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Brand:
CAT.NO. : ARG82693
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Product Details
内含物
| 货号 | 内含物名称 | 包装 | 温度 |
|---|---|---|---|
| ARG82693-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG82693-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG82693-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG82693-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82693-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82693-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82693-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82693-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG82693-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG82693-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG82693-011 | Plate sealer | 4 strips | Room temperature |
概述
| 产品描述 | ARG82693 Human MICA ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human MICA in serum and cell culture supernatants. |
|---|---|
| 反应物种 | Hu |
| 应用 | ELISA |
| 靶点名称 | MICA |
| 偶联标记 | HRP |
| 偶联标记说明 | Substrate: TMB and read at 450 nm. |
| 灵敏度 | 32 pg/ml |
| 样品类型 | Serum and cell culture supernatants. |
| 标准范围 | 62.5 - 4000 pg/ml |
| 样本量 | 100 µl |
| 精确 | Intra-Assay CV: 7.3% Inter-Assay CV: 8.1% |
| 別名 | MHC class I polypeptide-related sequence A; PERB11.1; MIC-A |
应用说明
| 检测时间 | ~ 5 hours |
|---|
属性
| 形式 | 96 well |
|---|---|
| 存放说明 | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | Swiss-port # Q29983 Human MHC class I polypeptide-related sequence A |
|---|---|
| 基因名称 | MICA |
| 全名 | MHC class I polypeptide-related sequence A |
| 背景介绍 | This gene encodes the highly polymorphic major histocompatability complex class I chain-related protein A. The protein product is expressed on the cell surface, although unlike canonical class I molecules it does not seem to associate with beta-2-microglobulin. It is a ligand for the NKG2-D type II integral membrane protein receptor. The protein functions as a stress-induced antigen that is broadly recognized by intestinal epithelial gamma delta T cells. Variations in this gene have been associated with susceptibility to psoriasis 1 and psoriatic arthritis, and the shedding of MICA-related antibodies and ligands is involved in the progression from monoclonal gammopathy of undetermined significance to multiple myeloma. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Jan 2014] |
| 生物功能 | Seems to have no role in antigen presentation. Acts as a stress-induced self-antigen that is recognized by gamma delta T-cells. Ligand for the KLRK1/NKG2D receptor. Binding to KLRK1 leads to cell lysis. [UniProt] |
| 细胞定位 | Cell membrane; Single-pass type I membrane protein. Cytoplasm. Note=Expressed on the cell surface in gastric epithelium, endothelial cells and fibroblasts and in the cytoplasm in keratinocytes and monocytes. Infection with human adenovirus 5 suppresses cell surface expression due to the adenoviral E3-19K protein which causes retention in the endoplasmic reticulum. [UniProt] |
| 产品亮点 | Related products: MICA antibodies; MICA ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| 翻译后修饰 | N-glycosylated. Glycosylation is not essential for interaction with KLRK1/NKG2D but enhances complex formation. Proteolytically cleaved and released from the cell surface of tumor cells which impairs KLRK1/NKG2D expression and T-cell activation. [UniProt] |
检测图片 (1)
ARG82693 Human MICA ELISA Kit standard curve image
ARG82693 Human MICA ELISA Kit results of a typical standard run with optical density reading at 450 nm.
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