Human MICB ELISA Kit

Protocol Booklet

Key features and details

Specification: 96 Test
Sensitivity: 1.09 pg/ml (50 μl)；6.68 pg/ml (10 μl)
Standard Curve Range: 6.86~5000 pg/ml
Standard Curve Gradient: 7 Points/3 Folds
Number of Incubations: 2
Sample Volume: 50 μl/10 μl
Assay type: Sandwich Elisa
Operation Duration: 120min

Brand:

CAT.NO. : AEH0127

RMB Please choose

Size:

96T

Trail, Bulk size or Custom requests Please contact us

*产品价格可能会有所调整，请以品牌方官网实时更新的价格为准，以确保准确性。

Product Details

Background

MICB (MHC class I chain-related gene B) is a transmembrane glycoprotein that functions as a ligand for NKG2D. A closely related protein, MICA, shares 85% amino acid identity with MICB. These 2 proteins are distantly related to the MHC class I proteins. MICA and MICB (MICA/B) possess three extracellular immunoglobulin-like domains, but have no capacity to bind peptide or interact with beta 2-microglobulin. The genes encoding MICA/B are found within the major histocompatibility complex on human chromosome 6. The MICB locus is polymorphic with more than 15 recognized human alleles. MICA/B are minimally expressed on normal cells, but are frequently expressed on epithelial tumors and can be induced by bacterial and viral infections. MICA/B are ligands for NKG2D, an activating receptor expressed on NK cells, NKT cells, gamma δ T cells, and CD8+ alpha beta T cells. Recognition of MICA/B by NKG2D results in the activation of cytolytic activity and/or cytokine production by these effector cells. MICA/B recognition is involved in tumor surveillance, viral infections, and autoimmune diseases. The release of soluble forms of MICA/B from tumors down-regulates NKG2D surface expression on effector cells resulting in the impairment of anti-tumor immune response

Typical data

pg/ml	O.D.		Average	Corrected
0.00	0.0208	0.0166	0.0187
6.86	0.0321	0.0300	0.0311	0.0124
20.58	0.0507	0.0474	0.0491	0.0304
61.73	0.0996	0.0959	0.0978	0.0791
185.19	0.2567	0.2492	0.2530	0.2343
555.56	0.7067	0.7088	0.7078	0.6891
1666.67	2.0300	1.9550	1.9925	1.9738
5000.00	3.8400	3.7960	3.8180	3.7993

Precision

	Intra-assay Precision			Inter-assay Precision
Sample Number	S1	S2	S3	S1	S2	S3
Sample Number	22	22	22	6	6	6
Average（pg/ml）	104.3	521.4	1862.1	99.0	528.2	1622.6
Standard deviation	4.2	23.5	101.7	8.2	28.2	62.3
Coefficient of variation（%）	4.1	4.5	5.5	5.2	5.3	3.8

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)Three samples of known concentration were tested six times on one plate to assess intra-assay precision.

Spike Recovery

The spike recovery was evaluated by spiking 3 levels of human MICB into health human serum sample. The un-spiked serum was used as blank in this experiment.

The recovery ranged from 84% to 108% with an overall mean recovery of 96%.

Sample Values

Sample Matrix

Sample Evaluated

Range (pg/ml)

Detectable

Mean of Detectable (pg/ml)

Serum

2.38-168.59

100.0%

45.33

Serum/Plasma - Thirty samples from apparently healthy volunteers were evaluated for the presence of human MICB in this assay.No medical histories were available for the donors.

New Products

Human Antithrombin III ELISA Kit

S100A7 Rabbit Polyclonal Antibody

SOX17 Mouse Monoclonal Antibody(ARA897)

SS18-SSX Rabbit Monoclonal Antibody(ARM647)

SOX9 Rabbit Monoclonal Antibody(ARA829)

Spectrin Rabbit Monoclonal Antibody(ARA894)