Human MICB ELISA Kit
Key features and details
- 产品描述:
- 反应物种:
- 应用:
- 特异性:
- 靶点名称:
- 偶联标记:
- 偶联标记说明:
- 灵敏度:
- 样品类型:
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Brand:
CAT.NO. : ARG81789
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Product Details
内含物
| 货号 | 内含物名称 | 包装 | 温度 |
|---|---|---|---|
| ARG81789-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81789-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81789-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81789-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81789-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81789-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81789-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81789-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81789-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81789-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81789-011 | Plate sealer | 4 strips | Room temperature |
概述
| 产品描述 | ARG81789 Human MICB ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human MICB in serum, plasma (heparin, EDTA) and cell culture supernatants. |
|---|---|
| 反应物种 | Hu |
| 应用 | ELISA |
| 特异性 | There is no detectable cross-reactivity with other relevant proteins. |
| 靶点名称 | MICB |
| 偶联标记 | HRP |
| 偶联标记说明 | Substrate: TMB and read at 450 nm. |
| 灵敏度 | 31.25 pg/ml |
| 样品类型 | Serum, plasma (heparin, EDTA) and cell culture supernatants. |
| 标准范围 | 62.5 - 4000 pg/ml |
| 样本量 | 100 µl |
| 精确 | Intra-Assay CV: 6.2%; Inter-Assay CV: 6.7% |
| 別名 | MHC class I polypeptide-related sequence B; MIC-B; PERB11.2 |
应用说明
| 检测时间 | ~ 5 hours |
|---|
属性
| 形式 | 96 well |
|---|---|
| 存放说明 | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | Swiss-port # Q29980 Human MHC class I polypeptide-related sequence B |
|---|---|
| 基因名称 | MICB |
| 全名 | MHC class I polypeptide-related sequence B |
| 背景介绍 | This gene encodes a heavily glycosylated protein which is a ligand for the NKG2D type II receptor. Binding of the ligand activates the cytolytic response of natural killer (NK) cells, CD8 alphabeta T cells, and gammadelta T cells which express the receptor. This protein is stress-induced and is similar to MHC class I molecules; however, it does not associate with beta-2-microglobulin or bind peptides. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014] |
| 生物功能 | Seems to have no role in antigen presentation. Acts as a stress-induced self-antigen that is recognized by gamma delta T cells. Ligand for the KLRK1/NKG2D receptor. Binding to KLRK1 leads to cell lysis. [UniProt] |
| 产品亮点 | Related products: MICB antibodies; MICB ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| 翻译后修饰 | Proteolytically cleaved and released from the cell surface of tumor cells. [UniProt] |
检测图片 (1)
ARG81789 Human MICB ELISA Kit standard curve image
ARG81789 Human MICB ELISA Kit results of a typical standard run with optical density reading at 450 nm.
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