Human MMP-13 ELISA Kit
Protocol Booklet
Key features and details
- Specification:
- Sensitivity:
- Standard Curve Range:
- Standard Curve Gradient:
- Number of Incubations:
- Sample Volume:
- Assay type:
- Operation Duration:
-
Brand:
Product Details
|
pg/ml |
O.D. |
Average |
Corrected |
|
|
0.00 |
0.0792 |
0.0843 |
0.0818 |
|
|
78.13 |
0.1480 |
0.1524 |
0.1502 |
0.0685 |
|
156.25 |
0.2363 |
0.2171 |
0.2267 |
0.1450 |
|
312.50 |
0.3585 |
0.3677 |
0.3631 |
0.2814 |
|
625.00 |
0.6069 |
0.6087 |
0.6078 |
0.5261 |
|
1250.00 |
1.1020 |
1.0940 |
1.0980 |
1.0163 |
|
2500.00 |
1.8900 |
1.9660 |
1.9280 |
1.8463 |
|
5000.00 |
3.3930 |
3.3560 |
3.3745 |
3.2928 |
|
Intra-assay Precision |
|
Inter-assay Precision |
|||||
|
Sample Number |
S1 |
S2 |
S3 |
S1 |
S2 |
S3 |
|
|
22 |
22 |
22 |
6 |
6 |
6 |
||
|
Average（pg/ml） |
56.9 |
372.0 |
1283.4 |
77.7 |
432.4 |
1499.5 |
|
|
Standard deviation |
3.3 |
22.3 |
74.8 |
2.8 |
13.2 |
38.0 |
|
|
Coefficient of variation（%） |
5.8 |
6.0 |
5.8 |
3.6 |
3.1 |
2.5 |
|
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
The recovery ranged from 92% to 117% with an overall mean recovery of 106%.
|
Sample Matrix |
Sample Evaluated |
Range (pg/ml) |
Detectable % |
Mean of Detectable (pg/ml) |
|
Serum |
30 |
5.75-44.20 |
100.0 |
21.14 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of MMP-13 in this assay. No medical histories were available for the donors.
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