Human MMP-8 ELISA Kit

Protocol Booklet

Key features and details

Specification: 96 Test
Sensitivity: 1.59 pg/ml (50 μl)；9.04 pg/ml (10 μl)
Standard Curve Range: 13.72~10000 pg/ml
Standard Curve Gradient: 7 Points/3 Folds
Number of Incubations: 2
Sample Volume: 50 μl/10 μl
Assay type: Sandwich Elisa
Operation Duration: 120min

Brand:

CAT.NO. : AEH0103

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Size:

96T

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Product Details

Background

The matrix metalloproteinases (MMPs) consist of 24 known human zinc proteases with essential roles in breaking down components of the extracellular matrix (ECM). Additional MMP substrates include cytokines, chemokines, growth factors and binding proteins, cell/cell adhesion molecules, and other proteinases. With a few exceptions, MMPs share common structural motifs including a pro-peptide domain, a catalytic domain, a hinge region, and a hemopexin-like domain. Synthesized as pro-enzymes, most MMPs are secreted before conversion to their active form. MMP activities are modulated on several levels including transcription, pro-enzyme activation, or by their endogenous inhibitors, tissue inhibitors of metalloproteinases (TIMPs). A subset of MMPs are associated with membranes and designated as membrane-type metalloproteinases (MT-MMP).

Typical Data

pg/ml	O.D.		Average	Corrected
0.00	0.0162	0.0172	0.0167
13.72	0.0324	0.0377	0.0351	0.0184
41.15	0.0650	0.0718	0.0684	0.0517
123.46	0.1828	0.1977	0.1903	0.1736
370.37	0.4943	0.5266	0.5105	0.4938
1111.11	1.1880	1.2360	1.2120	1.1953
3333.33	2.5860	2.9120	2.7490	2.7323
10000.00	4.2764	4.3073	4.2919	4.2752

Precision

	Intra-assay Precision			Inter-assay Precision
Sample Number	S1	S2	S3	S1	S2	S3
Sample Number	22	22	22	6	6	6
Average（pg/ml）	195.0	1021.4	3035.2	184.8	986.8	2963.6
Standard deviation	11	41.3	92.6	7.4	18.4	97.6
Coefficient of variation（%）	5.7	4.0	3.0	4.0	1.9	3.3

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)Three samples of known concentration were tested six times on one plate to assess intra-assay precision.

Spike Recovery

The spike recovery was evaluated by spiking 3 levels of human MMP-8 into health human serum sample. The un-spiked serum was used as blank in this experiment. The recovery ranged from 84% to 100% with an overall mean recovery of 91% .

Sample Values

Sample Matrix

Sample Evaluated

Range (ng/ml)

Detectable

Mean of Detectable (ng/ml)

Serum

4.84-76.92

100

23.05

Serum/Plasma - Thirty samples from apparently healthy volunteers were evaluated for the presence of human MMP-8 in this assay. No medical histories were available for the donors.

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