Human MMP-8 ELISA Kit
Protocol Booklet
Key features and details
- Specification:
- Sensitivity:
- Standard Curve Range:
- Standard Curve Gradient:
- Number of Incubations:
- Sample Volume:
- Assay type:
- Operation Duration:
-
Brand:
CAT.NO. : AEH0103
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Size:
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Product Details
Background
The matrix metalloproteinases (MMPs) consist of 24 known human zinc proteases with essential roles in breaking down components of the extracellular matrix (ECM). Additional MMP substrates include cytokines, chemokines, growth factors and binding proteins, cell/cell adhesion molecules, and other proteinases. With a few exceptions, MMPs share common structural motifs including a pro-peptide domain, a catalytic domain, a hinge region, and a hemopexin-like domain. Synthesized as pro-enzymes, most MMPs are secreted before conversion to their active form. MMP activities are modulated on several levels including transcription, pro-enzyme activation, or by their endogenous inhibitors, tissue inhibitors of metalloproteinases (TIMPs). A subset of MMPs are associated with membranes and designated as membrane-type metalloproteinases (MT-MMP).
Typical Data
|
pg/ml |
O.D. |
Average |
Corrected |
|
|
0.00 |
0.0162 |
0.0172 |
0.0167 |
|
|
13.72 |
0.0324 |
0.0377 |
0.0351 |
0.0184 |
|
41.15 |
0.0650 |
0.0718 |
0.0684 |
0.0517 |
|
123.46 |
0.1828 |
0.1977 |
0.1903 |
0.1736 |
|
370.37 |
0.4943 |
0.5266 |
0.5105 |
0.4938 |
|
1111.11 |
1.1880 |
1.2360 |
1.2120 |
1.1953 |
|
3333.33 |
2.5860 |
2.9120 |
2.7490 |
2.7323 |
|
10000.00 |
4.2764 |
4.3073 |
4.2919 |
4.2752 |
Precision
|
Intra-assay Precision |
Inter-assay Precision |
|||||
|
Sample Number |
S1 |
S2 |
S3 |
S1 |
S2 |
S3 |
|
22 |
22 |
22 |
6 |
6 |
6 |
|
|
Average（pg/ml） |
195.0 |
1021.4 |
3035.2 |
184.8 |
986.8 |
2963.6 |
|
Standard deviation |
11 |
41.3 |
92.6 |
7.4 |
18.4 |
97.6 |
|
Coefficient of variation（%） |
5.7 |
4.0 |
3.0 |
4.0 |
1.9 |
3.3 |
- Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
- Inter-assay Precision (Precision between assays)Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human MMP-8 into health human serum sample. The un-spiked serum was used as blank in this experiment. The recovery ranged from 84% to 100% with an overall mean recovery of 91% .
Sample Values
|
Sample Matrix |
Sample Evaluated |
Range (ng/ml) |
Detectable % |
Mean of Detectable (ng/ml) |
|
Serum |
30 |
4.84-76.92 |
100 |
23.05 |
Serum/Plasma - Thirty samples from apparently healthy volunteers were evaluated for the presence of human MMP-8 in this assay. No medical histories were available for the donors.
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