Human MMP1 (Total) ELISA kit
Key features and details
- 产品描述:
- 反应物种:
- 应用:
- 特异性:
- 靶点名称:
- 偶联标记:
- 偶联标记说明:
- 灵敏度:
- 样品类型:
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Brand:
CAT.NO. : ARG80145
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Product Details
内含物
| 货号 | 内含物名称 | 包装 | 温度 |
|---|---|---|---|
| ARG80145-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG80145-002 | Standard (Lyophilized) | 3 X 2 ng/vial | 4°C |
| ARG80145-003 | Standard diluent buffer | 20 ml | 4°C |
| ARG80145-004 | Antibody conjugate concentrate | 400 µl | 4°C |
| ARG80145-005 | Antibody diluent buffer | 16 ml | 4°C |
| ARG80145-006 | HRP-Streptavidin concentrate | 400 µl | 4°C (Protect from light) |
| ARG80145-007 | HRP-Streptavidin diluent buffer | 16 ml | 4°C |
| ARG80145-008 | 20X Wash buffer | 50 ml | 4°C |
| ARG80145-009 | TMB substrate | 12ml | 4°C (Protect from light) |
| ARG80145-010 | STOP solution | 12ml | 4°C |
| ARG80145-011 | Plate sealer | 4 strips | Room temperature |
概述
| 产品描述 | ARG80145 Human MMP1 (Total) ELISA kit is an Enzyme Immunoassay kit for the quantification of Human MMP1 (Total) in Serum, Plasma, Cell culture supernatants. |
|---|---|
| 反应物种 | Hu |
| 应用 | ELISA |
| 特异性 | No significant cross-reactivity or interference with Human MMP-2,3,7,8,9,10,12,13,16, TIMP-1,2,3,4 |
| 靶点名称 | MMP1 |
| 偶联标记 | HRP |
| 偶联标记说明 | Substrate: TMB and read at 450 nm |
| 灵敏度 | 30 pg/ml |
| 样品类型 | Serum, Plasma, Cell culture supernatants |
| 标准范围 | 62.5 - 4000 pg/ml |
| 样本量 | 100 µl |
| 精确 | CV: less than10% |
| 別名 | MMP-1; CLG; Fibroblast collagenase; Matrix metalloproteinase-1; CLGN; EC 3.4.24.7; Interstitial collagenase |
应用说明
| 检测时间 | 4 hours |
|---|
属性
| 形式 | 96 well |
|---|---|
| 存放说明 | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | MMP1 |
| 全名 | matrix metallopeptidase 1 |
| 背景介绍 | The matrix metalloproteinases (MMPs) are a family of at least eighteen secreted and membrane-bound zincendopeptidases. Collectively, these enzymes can degrade all the components of the extracellular matrix, including fibrillar and non-fibrillar collagens, fibronectin, laminin and basement membrane glycoproteins. In general, a signal peptide, a propeptide, and a catalytic domain containing the highly conserved zinc-binding site characterizes the structure of the MMPs. In addition, fibronectin-like repeats, a hinge region, and a C-terminal hemopexin-like domain allow categorization of MMPs into the collagenase, gelatinase, stomelysin and membrane-type MMP subfamilies. All MMPs are synthesized as proenzymes, and most of them are secreted from the cells as proenzymes. Thus, the activation of these proenzymes is a critical step that leads to extracellular matrix breakdown. MMPs are considered to play an important role in wound healing, apoptosis, bone elongation, embryo development, uterine involution, angiogenesis and tissue remodeling, and in diseases such as multiple sclerosis, Alzheimer's, malignant gliomas, lupus, arthritis, periodontis, glumerulonephritis, atherosclerosis, tissue ulceration, and in cancer cell invasion and metastasis. |
| 产品亮点 | Related products: MMP1 antibodies; MMP1 ELISA Kits; Related news: Detecting MMPs and their non-ECM substrates New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| 研究领域 | Cancer kit; Cell Biology and Cellular Response kit; Signaling Transduction kit |
| 翻译后修饰 | Undergoes autolytic cleavage to two major forms (22 kDa and 27 kDa). A minor form (25 kDa) is the glycosylated form of the 22 kDa form. The 27 kDa form has no activity while the 22/25 kDa form can act as activator for collagenase. Tyrosine phosphorylated in platelets by PKDCC/VLK. |
检测图片 (1)
ARG80145 Human MMP1 (Total) ELISA kit standard curve image
ARG80145 Human MMP1 (Total) ELISA kit results of a typical standard run with optical density reading at 450 nm.
参考文献
Adipose-derived stem cells enriched with therapeutic mRNA TGF-β3 and IL-10 synergistically promote scar-less wound healing in preclinical models
ELISA / Human / Culture supernatant
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