Human MMP7 (active) ELISA Kit
Key features and details
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- 反应物种:
- 应用:
- 特异性:
- 靶点名称:
- 偶联标记:
- 偶联标记说明:
- 灵敏度:
- 样品类型:
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Brand:
CAT.NO. : ARG82010
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Product Details
内含物
| 货号 | 内含物名称 | 包装 | 温度 |
|---|---|---|---|
| ARG82010-001 | Antibody-coated microplate | 12 X 8 strips | 4°C |
| ARG82010-002 | Standard (156 ng) | 1 vial | 4°C, store at -20°C after reconstitution |
| ARG82010-003 | Standard reconstitution buffer | 1.1 ml | 4°C |
| ARG82010-004 | HRP-antibody Conjugate (200X) | 65 µl | 4°C |
| ARG82010-005 | Sample diluent | 15 ml | 4°C |
| ARG82010-006 | Assay buffer | 12 ml | 4°C |
| ARG82010-007 | Antibody diluent | 12 ml | 4°C |
| ARG82010-008 | 10X Wash buffer | 30 ml | 4°C |
| ARG82010-009 | TMB substrate | 12 ml (ready-to-use) | 4°C (Protect from light) |
| ARG82010-010 | Stop solution | 6 ml | 4°C |
| ARG82010-011 | Plate sealer | 1 strip | Room temperature |
概述
| 产品描述 | ARG82010 Human MMP7 (active) ELISA Kit is an Enzyme Immunoassay kit for the quantification of human MMP7 active form protein in serum, plasma, saliva, urine and cell culture supernatants. |
|---|---|
| 反应物种 | Hu |
| 应用 | ELISA |
| 特异性 | No specific signal was detected when using pro-MMP7 recombinant protein (20 ng/ml) |
| 靶点名称 | MMP7 (active) |
| 偶联标记 | HRP |
| 偶联标记说明 | Substrate: TMB and read at 450 nm. |
| 灵敏度 | 0.102 ng/ml |
| 样品类型 | Serum, plasma, saliva, urine and cell culture supernatants. |
| 标准范围 | 0.3125 - 20 ng/ml |
| 样本量 | 100 µl |
| 精确 | Intra-Assay CV: 8.9%; Inter-Assay CV: 7.6% |
| 別名 | MMP-7; Uterine metalloproteinase; Matrix metalloproteinase-7; EC 3.4.24.23; Matrilysin; Matrin; Pump-1 protease; PUMP-1; MPSL1 |
应用说明
| 检测时间 | ~ 4 hours |
|---|
属性
| 形式 | 96 well |
|---|---|
| 存放说明 | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | MMP7 |
| 全名 | matrix metallopeptidase 7 |
| 背景介绍 | MMP7 is a member of the peptidase M10 family of matrix metalloproteinases (MMPs). Proteins in this family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. The encoded preproprotein is proteolytically processed to generate the mature protease. This secreted protease breaks down proteoglycans, fibronectin, elastin and casein and differs from most MMP family members in that it lacks a conserved C-terminal hemopexin domain. The enzyme is involved in wound healing, and studies in mice suggest that it regulates the activity of defensins in intestinal mucosa. The gene is part of a cluster of MMP genes on chromosome 11. This gene exhibits elevated expression levels in multiple human cancers. [provided by RefSeq, Jan 2016] |
| 生物功能 | MMP7 degrades casein, gelatins of types I, III, IV, and V, and fibronectin. Activates procollagenase. [UniProt] |
| 细胞定位 | Secreted, extracellular space, extracellular matrix. [UniProt] |
| 产品亮点 | Related products: MMP7 antibodies; MMP7 ELISA Kits; Related news: New MMP7 (total or active) ELISA kits are released Detecting MMPs and their non-ECM substrates New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
检测图片 (2)
ARG82010 Human MMP7 (active) ELISA Kit standard curve image
ARG82010 Human MMP7 (active) ELISA Kit results of a typical standard run with optical density reading at 450 nm.
ARG82010 Human MMP7 (active) ELISA Kit standard curve image
ARG82010 Human MMP7 (active) ELISA Kit results of a typical standard run with optical density reading at 450 nm. Red: Active MMP7; Green: Pro-MMP7.
参考文献
Crosstalk between E-Cadherin/β-Catenin and NF-κB Signaling Pathways: The Regulation of Host-Pathogen Interaction during Leptospirosis
ELISA / Human / Cell culture supernatants
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