Human/Mouse BDNF ELISA Kit

Key features and details

Specification: 96 Test
Sensitivity: 1.05 pg/ml (50 μl)；2.53 pg/ml (10 μl)
Standard Curve Range: 1.37~1000 pg/ml
Standard Curve Gradient: 7 Points/3 Folds
Number of Incubations: 2
Sample Volume: 50 μl/10 μl
Assay type: Sandwich Elisa
Operation Duration: 120min

Brand:

CAT.NO. : AEH0069

RMB Please choose

Size:

96T

Trail, Bulk size or Custom requests Please contact us

*产品价格可能会有所调整，请以品牌方官网实时更新的价格为准，以确保准确性。

Product Details

Background

BDNF (Brain-Derived Neurotrophic Factor) is a secreted protein that regulates many aspects of neuronal development and function in the nervous system. It is produced as proBDNF in response to neuronal activity or inflammatory stimulation and is then cleaved before associating into a homodimer. It is expressed throughout the nervous system as well as by fibroblasts, megakaryocytes/platelets, and smooth muscle cells. BDNF regulates neural stem cell survival and differentiation, axon/dendrite differentiation, synapse formation and maturation, and refinement of developing circuits. It is also a key regulator of synaptic plasticity and late-phase long-term potentiation. BDNF signals through the TrkB and NGF R/TNFRSF16 receptors. Secreted proBDNF can bind to NGF R/TNFRSF16 and induce long-term depression and cell apoptosis.

Typical Data

pg/ml	O.D.		Average	Corrected
0.00	0.0195	0.0195	0.0195
1.37	0.0241	0.0238	0.0240	0.0045
4.12	0.0410	0.0387	0.0399	0.0204
12.35	0.0961	0.0886	0.0924	0.0729
37.04	0.2670	0.2588	0.2629	0.2434
111.11	0.7864	0.7770	0.7817	0.7622
333.33	1.9080	1.9160	1.9120	1.8925
1000.00	3.3430	3.3080	3.3255	3.3060

Precision

	Intra-assay Precision			Inter-assay Precision
Sample Number	S1	S2	S3	S1	S2	S3
Sample Number	22	22	22	6	6	6
Average(pg/ml)	26.4	132.7	428.3	26.0	132.0	417.2
Standard Deviation	0.9	5.5	28.5	0.8	5.2	22.7
Coefficient of Variation(%)	3.4	4.1	6.7	3.1	4.0	5.4

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.

Spike Recovery

The spike recover y was evaluated by spiking 3 levels of human BDNF into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 90% to 118% with an overall mean recovery of 104%.

Sample Values

Sample Matrix	Sample Evaluated	Range (ng/ml)	Detectable (%)	Mean of Detectable (ng/ml)
Serum	30	12.32-21.28	100	16.8

Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of BDNF in this assay. No medical histories were available for the donors.

New Products

Human Antithrombin III ELISA Kit

S100A7 Rabbit Polyclonal Antibody

SOX17 Mouse Monoclonal Antibody(ARA897)

SS18-SSX Rabbit Monoclonal Antibody(ARM647)

SOX9 Rabbit Monoclonal Antibody(ARA829)

Spectrin Rabbit Monoclonal Antibody(ARA894)