Human NCAM 1/CD56 ELISA Kit
Key features and details
- Specification:
- Sensitivity:
- Standard Curve Range:
- Standard Curve Gradient:
- Number of Incubations:
- Sample Volume:
- Assay type:
- Operation Duration:
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Brand:
Product Details
Neural cell adhesion molecule 1 (NCAM-1) is a multifunctional member of the Ig superfamily. It belongs to a family of membrane-bound glycoproteins that are involved in Ca++ independent cell matrix and homophilic or heterophilic cell-cell interactions. NCAM-1 specifically binds to heparan sulfate proteoglycans, the extracellular matrix protein agrin, and several chondroitin sulfate proteoglycans that include neurocan and phosphocan. There are three main forms of human NCAM-1 that arise by alternate splicing. These are designated NCAM-120/NCAM-1 (761 amino acids [aa]), NCAM-140 (848 aa), and NCAM-180 (1120 aa). NCAM-120 is GPI-linked, while NCAM-140 and NCAM-180 are type I transmembrane glycoproteins. Additional alternate splicing adds considerable diversity to all three forms, and extracellular proteolytic processing is possible for NCAM-180. NCAM-1 is synthesized as a 761 aa preproprecursor that contains a 19 aa signal sequence, a 722 aa GPI-linked mature region, and a 20 aa C-terminal prosegment. The molecule contains five C-2 type Ig-like domains and two fibronectin type-III domains. Human to mouse, NCAM-1 is 93% aa identical. NCAM-1 appears to be highly sialylated. The polysialyation of NCAM-1 reduces its adhesive property and increases its neurite outgrowth promoting features. NCAM-1 in the adult brain shows a decline of sialylation relative to earlier developmental periods. In regions that retain a high degree of neuronal plasticity, however, the adult brain continues to express polysialylation-NCAM-1, suggesting sialylation of NCAM-1 is involved in regenerative processes and synaptic plasticity.
|
ng/ml |
O.D. |
Average |
Corrected |
|
|
0.00 |
0.0268 |
0.0287 |
0.0278 |
|
|
0.03 |
0.0416 |
0.0360 |
0.0388 |
0.0111 |
|
0.08 |
0.0543 |
0.0660 |
0.0602 |
0.0324 |
|
0.25 |
0.1195 |
0.1114 |
0.1155 |
0.0877 |
|
0.74 |
0.2833 |
0.2774 |
0.2804 |
0.2526 |
|
2.22 |
0.6849 |
0.7219 |
0.7034 |
0.6757 |
|
6.67 |
1.9060 |
1.9530 |
1.9295 |
1.9018 |
|
20.00 |
3.7930 |
3.9828 |
3.8879 |
3.8602 |
|
|
Inter-assay Precision |
|||||
|
Sample Number |
S1 |
S2 |
S3 |
S1 |
S2 |
S3 |
|
22 |
22 |
22 |
6 |
6 |
6 |
|
|
Average(pg/ml) |
233.5 |
1498.4 |
4930.0 |
366.5 |
1837.9 |
5636.7 |
|
Standard Deviation |
18.1 |
103.1 |
326.6 |
10.0 |
52.2 |
150.9 |
|
Coefficient of Variation(%) |
7.8 |
6.9 |
6.6 |
2.7 |
2.8 |
2.7 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
The recovery ranged from 80% to 98% with an overall mean recovery of 88%.
|
Sample Matrix |
Sample Evaluated |
Range (ng/ml) |
Detectable (%) |
Mean of Detectable (ng/ml) |
|
Serum |
30 |
286.3-459.6 |
100 |
370.6 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of NCAM-1/CD56 in this assay. No medical histories were available for the donors.
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