Human NECTIN4 / PVRL4 ELISA Kit
Key features and details
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- 应用:
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- 靶点名称:
- 偶联标记:
- 偶联标记说明:
- 灵敏度:
- 样品类型:
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Brand:
CAT.NO. : ARG81602
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Product Details
内含物
| 货号 | 内含物名称 | 包装 | 温度 |
|---|---|---|---|
| ARG81602-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81602-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81602-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81602-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81602-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81602-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81602-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81602-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81602-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81602-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81602-011 | Plate sealer | 4 strips | Room temperature |
概述
| 产品描述 | ARG81602 Human NECTIN4 / PVRL4 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human NECTIN4 / PVRL4 in serum, plasma (heparin, EDTA), urine and cell culture supernatants. |
|---|---|
| 反应物种 | Hu |
| 应用 | ELISA |
| 特异性 | There is no detectable cross-reactivity with other relevant proteins. |
| 靶点名称 | NECTIN4 / PVRL4 |
| 偶联标记 | HRP |
| 偶联标记说明 | Substrate: TMB and read at 450 nm. |
| 灵敏度 | 15.6 pg/ml |
| 样品类型 | Serum, plasma (heparin, EDTA), urine and cell culture supernatants. |
| 标准范围 | 31.2 - 2000 pg/ml |
| 样本量 | 100 µl |
| 精确 | Intra-Assay CV: 5.5% Inter-Assay CV: 6.1% |
| 別名 | Nectin-4; Ig superfamily receptor LNIR; PRR4; LNIR; nectin-4; EDSS1; Poliovirus receptor-related protein 4; Nectin cell adhesion molecule 4 |
应用说明
| 检测时间 | ~ 5 hours |
|---|
属性
| 形式 | 96 well |
|---|---|
| 存放说明 | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | PVRL4 |
| 全名 | poliovirus receptor-related 4 |
| 背景介绍 | This gene encodes a member of the nectin family. The encoded protein contains two immunoglobulin-like (Ig-like) C2-type domains and one Ig-like V-type domain. It is involved in cell adhesion through trans-homophilic and -heterophilic interactions. It is a single-pass type I membrane protein. The soluble form is produced by proteolytic cleavage at the cell surface by the metalloproteinase ADAM17/TACE. The secreted form is found in both breast tumor cell lines and breast tumor patients. Mutations in this gene are the cause of ectodermal dysplasia-syndactyly syndrome type 1, an autosomal recessive disorder. Alternatively spliced transcript variants have been found but the full-length nature of the variant has not been determined.[provided by RefSeq, Jan 2011] |
| 生物功能 | Seems to be involved in cell adhesion through trans-homophilic and -heterophilic interactions, the latter including specifically interactions with PVRL2/nectin-1. Does not act as receptor for alpha-herpesvirus entry into cells. [UniProt] |
| 细胞定位 | Cell membrane; Single-pass type I membrane protein. Cell junction, adherens junction. Note=Colocalizes with AFDN at cadherin-based adherens junctions (PubMed:11544254). Processed poliovirus receptor-related protein 4: Secreted. Note=The secreted form is found in breast tumor patients (PubMed:15784625). [UniProt] |
| 产品亮点 | New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| 翻译后修饰 | The soluble form is produced by proteolytic cleavage at the cell surface (shedding), probably by ADAM17/TACE. [UniProt] |
检测图片 (1)
ARG81602 Human NECTIN4 / PVRL4 ELISA Kit standard curve image
ARG81602 Human NECTIN4 / PVRL4 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
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