Human Neutrophil Elastase ELISA Kit
Key features and details
- 产品描述:
- 反应物种:
- 应用:
- 靶点名称:
- 偶联标记:
- 偶联标记说明:
- 灵敏度:
- 样品类型:
- 标准范围:
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Brand:
CAT.NO. : ARG82775
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Product Details
内含物
| 货号 | 内含物名称 | 包装 | 温度 |
|---|---|---|---|
| ARG82775-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG82775-002 | Standard | 2 X 50 ng/vial | 4°C |
| ARG82775-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG82775-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82775-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82775-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82775-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82775-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG82775-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG82775-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG82775-011 | Plate sealer | 4 strips | Room temperature |
概述
| 产品描述 | ARG82775 Human Neutrophil Elastase ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human Neutrophil Elastase in serum, plasma (EDTA, heparin) and cell culture supernatants. |
|---|---|
| 反应物种 | Hu |
| 应用 | ELISA |
| 靶点名称 | Neutrophil Elastase |
| 偶联标记 | HRP |
| 偶联标记说明 | Substrate: TMB and read at 450 nm. |
| 灵敏度 | 0.39 ng/ml |
| 样品类型 | Serum, plasma (EDTA, heparin) and cell culture supernatants. |
| 标准范围 | 0.78 - 50 ng/ml |
| 样本量 | 100 µl |
| 精确 | Intra-Assay CV: 4.3% Inter-Assay CV: 5.3% |
| 別名 | Neutrophil elastase; HNE; EC 3.4.21.37; Medullasin; NE; ELA2; GE; Bone marrow serine protease; SCN1; PMN-E; Human leukocyte elastase; HLE; Elastase-2; PMN elastase |
应用说明
| 检测时间 | ~ 5 hours |
|---|
属性
| 形式 | 96 well |
|---|---|
| 存放说明 | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | ELANE |
| 全名 | elastase, neutrophil expressed |
| 背景介绍 | Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode structurally similar proteins. The encoded preproprotein is proteolytically processed to generate the active protease. Following activation, this protease hydrolyzes proteins within specialized neutrophil lysosomes, called azurophil granules, as well as proteins of the extracellular matrix. The enzyme may play a role in degenerative and inflammatory diseases through proteolysis of collagen-IV and elastin. This protein also degrades the outer membrane protein A (OmpA) of E. coli as well as the virulence factors of such bacteria as Shigella, Salmonella and Yersinia. Mutations in this gene are associated with cyclic neutropenia and severe congenital neutropenia (SCN). This gene is present in a gene cluster on chromosome 19. [provided by RefSeq, Jan 2016] |
| 生物功能 | Modifies the functions of natural killer cells, monocytes and granulocytes. Inhibits C5a-dependent neutrophil enzyme release and chemotaxis (PubMed:15140022). Capable of killing E.coli but not S.aureus in vitro; digests outer membrane protein A (ompA) in E.coli and K.pneumoniae (PubMed:10947984). [UniProt] |
检测图片 (1)
ARG82775 Human Neutrophil Elastase ELISA Kit standard curve image
ARG82775 Human Neutrophil Elastase ELISA Kit results of a typical standard run with optical density reading at 450 nm.
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