Human Pentraxin 3/PTX3 ELISA Kit

Protocol Booklet

Key features and details

Specification: 96 Test
Sensitivity: 1.99 pg/ml (50 μl)；9.53 pg/ml (10 μl)
Standard Curve Range: 13.73~10000 pg/ml
Standard Curve Gradient: 7 Points/3 Folds
Number of Incubations: 2
Sample Volume: 50 μl/10 μl
Assay type: Sandwich Elisa
Operation Duration: 120min

Brand:

CAT.NO. : AEH0141

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Size:

96T

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Product Details

Background

Pentraxin 3 (PTX3), also known as TNF-stimulated gene 14 (TSG-14), is a secreted glycoprotein belonging to the Pentraxin family of proteins. It is the prototypical long Pentraxin, exhibiting a C-terminal Pentraxin domain characteristic of the family, and a unique N-terminal domain. It is produced by several cell types including endothelial and mononuclear cells. PTX3 acts as a pattern recognition receptor with non-redundant roles in the innate immune response to several microbial agents including the fungal pathogen Aspergillus fumigatus. Overexpression leads to enhanced pro-inflammatory responses. PTX3 is induced in response to LPS and inflammatory cytokines including TNF-alpha and IL-1 beta.

typical data

pg/ml	O.D.		Average	Corrected
0.00	0.0431	0.0393	0.0412
13.72	0.0704	0.0717	0.0711	0.0299
41.15	0.1370	0.1360	0.1365	0.0953
123.46	0.3247	0.3126	0.3187	0.2775
370.37	0.8276	0.7558	0.7917	0.7505
1111.11	1.7600	1.6140	1.6870	1.6458
3333.33	3.0380	2.8520	2.9450	2.9038
10000.00	3.8390	3.6580	3.7485	3.7073

Precision

	Intra-assay Precision			Inter-assay Precision
Sample Number	S1	S2	S3	S1	S2	S3
Sample Number	22	22	22	6	6	6
Average（pg/ml）	213.5	1031.5	3290.1	242.9	1110.6	3753.9
Standard deviation	11.3	63.9	223.3	5.3	51.7	224.3
Coefficient of variation（%）	5.3	6.2	6.8	2.2	4.7	6.0

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.

Spike Recovery

The spike recovery was evaluated by spiking 3 levels of human Pentraxin 3/PTX3 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 80% to 118% with an overall mean recovery of 104%.

Sample Values

Sample Matrix

Sample

Evaluated

Range

(pg/ml)

Detectable

Mean of Detectable

(pg/ml)

Serum

1308.85-6892.74

100

3254.30

Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of Pentraxin 3/PTX3 in this assay. No medical histories were available for the donors.

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