Human Peroxiredoxin 2 ELISA Kit
Key features and details
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- 反应物种:
- 应用:
- 特异性:
- 靶点名称:
- 偶联标记:
- 偶联标记说明:
- 灵敏度:
- 样品类型:
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Brand:
CAT.NO. : ARG82038
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Product Details
内含物
| 货号 | 内含物名称 | 包装 | 温度 |
|---|---|---|---|
| ARG82038-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG82038-002 | Standard | 2 X 20 ng/vial | 4°C |
| ARG82038-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG82038-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82038-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82038-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82038-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82038-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG82038-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG82038-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG82038-011 | Plate sealer | 4 strips | Room temperature |
概述
| 产品描述 | ARG82038 Human Peroxiredoxin 2 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human Peroxiredoxin 2 in serum, plasma (heparin, EDTA) and cell culture supernatants. |
|---|---|
| 反应物种 | Hu |
| 应用 | ELISA |
| 特异性 | Cross Reactivity: Human Recombinant Peroxiredoxin 1: 0.2% Human Recombinant Peroxiredoxin 3: 5.1% Human Recombinant Peroxiredoxin 4: 19.8% Human Recombinant Peroxiredoxin 5: 0.3% |
| 靶点名称 | Peroxiredoxin 2 |
| 偶联标记 | HRP |
| 偶联标记说明 | Substrate: TMB and read at 450 nm. |
| 灵敏度 | 156 pg/ml |
| 样品类型 | Serum, plasma (heparin, EDTA) and cell culture supernatants. |
| 标准范围 | 312 - 20000 pg/ml |
| 样本量 | 100 µl |
| 精确 | Intra-Assay CV: 5.6%; Inter-Assay CV: 6.6% |
| 別名 | EC 1.11.1.15; TPX1; TDPX1; HEL-S-2a; PTX1; Thiol-specific antioxidant protein; PRX2; TSA; Peroxiredoxin-2; PRP; Thioredoxin-dependent peroxide reductase 1; NKEF-B; NKEFB; Natural killer cell-enhancing factor B; Thioredoxin peroxidase 1; PRXII |
应用说明
| 检测时间 | ~ 5 hours |
|---|
属性
| 形式 | 96 well |
|---|---|
| 存放说明 | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | PRDX2 |
| 全名 | peroxiredoxin 2 |
| 背景介绍 | This gene encodes a member of the peroxiredoxin family of antioxidant enzymes, which reduce hydrogen peroxide and alkyl hydroperoxides. The encoded protein plays an antioxidant protective role in cells, and it may contribute to the antiviral activity of CD8(+) T-cells. The crystal structure of this protein has been resolved to 2.7 angstroms. This protein prevents hemolytic anemia from oxidative stress by stabilizing hemoglobin, thus making this gene a therapeutic target for patients with hemolytic anemia. This protein may have a proliferative effect and play a role in cancer development or progression. Related pseudogenes have been identified on chromosomes 5, 6, 10 and 13. [provided by RefSeq, Mar 2013] |
| 生物功能 | Involved in redox regulation of the cell. Reduces peroxides with reducing equivalents provided through the thioredoxin system. It is not able to receive electrons from glutaredoxin. May play an important role in eliminating peroxides generated during metabolism. Might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H(2)O(2). [UniProt] |
| 细胞定位 | Cytoplasm. [UniProt] |
| 产品亮点 | Related products: Peroxiredoxin antibodies; Peroxiredoxin ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
检测图片 (1)
ARG82038 Human Peroxiredoxin 2 ELISA Kit standard curve image
ARG82038 Human Peroxiredoxin 2 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
参考文献
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