Human PLA2R ELISA Kit
Key features and details
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- 靶点名称:
- 偶联标记:
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CAT.NO. : ARG82686
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Product Details
概述
| 产品描述 | ARG82686 Human PLA2R ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human PLA2R in serum and plasma. |
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| 反应物种 | Hu |
| 应用 | ELISA |
| 特异性 | The following recombinant Human proteins were tested and exhibited no cross-reactivity or interference: ApoAI, BMP1, BMP2, BMP3, BMP4, BMP7, CRP, HGF, HSP27, IL1 alpha, IL1 RI, IL1 beta, IL1RA, IL2, IL4, IL5, IL6, IL8, IL10, IL12, IL13, IL15, IL17C, IL21, IL23, IFN gamma, MMP2, sIL2R, sIL6R, PDGF, PLA2G7, prolactin, TGF beta 1, TGF beta 2, TGF beta 3, TLR1, TLR2, TLR3, TNF alpha, TNF RI, TNF RII and VEGF. |
| 靶点名称 | PLA2R |
| 偶联标记 | HRP |
| 偶联标记说明 | Read at 450 nm. |
| 灵敏度 | 78 pg/ml |
| 样品类型 | Serum and plasma |
| 标准范围 | 156 - 10000 pg/ml |
| 样本量 | 100 µl |
| 精确 | Intra-Assay CV: 6% Inter-Assay CV: 9% |
| 別名 | M-type receptor; Soluble PLA2-R; 180 kDa secretory phospholipase A2 receptor; Soluble PLA2R; PLA2G1R; CLEC13C; PLA2-R; C-type lectin domain family 13 member C; PLA2R; Secretory phospholipase A2 receptor; PLA2IR |
应用说明
| 检测时间 | ~ 3 hours |
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属性
| 形式 | 96 well |
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| 存放说明 | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | Swiss-port # Q13018 Human Secretory phospholipase A2 receptor |
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| 基因名称 | PLA2R1 |
| 全名 | phospholipase A2 receptor 1, 180kDa |
| 背景介绍 | This gene represents a phospholipase A2 receptor. The encoded protein likely exists as both a transmembrane form and a soluble form. The transmembrane receptor may play a role in clearance of phospholipase A2, thereby inhibiting its action. Polymorphisms at this locus have been associated with susceptibility to idiopathic membranous nephropathy. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Sep 2010] |
| 生物功能 | Receptor for secretory phospholipase A2 (sPLA2). Acts as a receptor for phospholipase sPLA2-IB/PLA2G1B but not sPLA2-IIA/PLA2G2A. Also able to bind to snake PA2-like toxins. Although its precise function remains unclear, binding of sPLA2 to its receptor participates in both positive and negative regulation of sPLA2 functions as well as clearance of sPLA2. Binding of sPLA2-IB/PLA2G1B induces various effects depending on the cell type, such as activation of the mitogen-activated protein kinase (MAPK) cascade to induce cell proliferation, the production of lipid mediators, selective release of arachidonic acid in bone marrow-derived mast cells. In neutrophils, binding of sPLA2-IB/PLA2G1B can activate p38 MAPK to stimulate elastase release and cell adhesion. May be involved in responses in proinflammatory cytokine productions during endotoxic shock. Also has endocytic properties and rapidly internalizes sPLA2 ligands, which is particularly important for the clearance of extracellular sPLA2s to protect their potent enzymatic activities. The soluble secretory phospholipase A2 receptor form is circulating and acts as a negative regulator of sPLA2 functions by blocking the biological functions of sPLA2-IB/PLA2G1B. [UniProt] |
| 细胞定位 | Cell membrane; Single-pass type I membrane protein. Soluble secretory phospholipase A2 receptor: Secreted. Isoform 2: Secreted. [UniProt] |
| 产品亮点 | Related products: PLA2R antibodies; PLA2R ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| 翻译后修饰 | The secretory phospholipase A2 receptor form may be produced by the action of metalloproteinases. It contains all extracellular domains and only lacks transmembrane and cytosolic regions. It is however unclear whether this form is produced by proteolytic cleavage as suggested by some experiments, or by alternative splicing, as in the case of isoform 2 that shares all characteristics of secretory phospholipase A2 receptor form (By similarity). [UniProt] |
检测图片 (1)
ARG82686 Human PLA2R ELISA Kit standard curve image
ARG82686 Human PLA2R ELISA Kit results of a typical standard run with optical density reading at 450 nm.
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