Human Prolactin ELISA Kit

Key features and details

Specification: 96 Test
Sensitivity: 3.47 pg/ml (50 μl)；11.20 pg/ml (10 μl)
Standard Curve Range: 31.25~2000 pg/ml
Standard Curve Gradient: 7 Points/3 Folds
Number of Incubations: 2
Sample Volume: 50 μl
Assay type: Sandwich Elisa
Operation Duration: 120min

Brand:

CAT.NO. : AEH0089

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Size:

96T

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Product Details

Background

Prolactin (PRL) is a neuroendocrine pituitary hormone that is synthesized by anterior pituitary, placenta, brain, uterus, dermal fibroblasts, decidua, B cells, T cells, natural killer cells, and breast cancer cells. The prolactin receptor is a transmembrane type I glycoprotein that belongs to the hematopoietic cytokine receptor family.

Prolactin receptor (PRL R) contains an extracellular, transmembrane, and intracellular domain. Transcriptional regulation of the PRL R gene results in several different species-dependent isoforms of PRL R being produced.

Tipical Data

pg/ml	O.D.		Average	Corrected
0	0.0672	0.063	0.0651
31.25	0.1314	0.1212	0.1263	0.0612
62.5	0.1703	0.1765	0.1734	0.1083
125	0.3135	0.3042	0.3089	0.2438
250	0.5341	0.5419	0.538	0.4729
500	1.022	1.01	1.016	0.9509
1000	1.916	1.937	1.9265	1.8614
2000	3.355	3.438	3.3965	3.3314

Precision

	Intra-assay Precision			Inter-assay Precision
Sample Number	S1	S2	S3	S1	S2	S3
Sample Number	22	22	22	6	6	6
Average(pg/ml)	38.9	216.2	558.7	43.9	229.2	598.9
Standard Deviation	3.2	6.4	16.7	3.3	8.4	18.1
Coefficient of Variation(%)	8	2.9	3	7.4	3.7	3

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.

Spike Recovery

The spike recovery was evaluated by spiking 3 levels of human Prolactin into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 81% to 115% with an overall mean recovery of 102%.

Sample Values

Sample Matrix	Sample Evaluated	Range (pg/ml)	Detectable (%)	Mean of Detectable (pg/ml)
Serum	30	1055.28-7606.60	100	3687.09

Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of Prolactin in this assay. No medical histories were available for the donors.

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