Human PZP / Pregnancy zone protein ELISA Kit
Key features and details
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- 反应物种:
- 应用:
- 靶点名称:
- 偶联标记:
- 偶联标记说明:
- 灵敏度:
- 样品类型:
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Brand:
CAT.NO. : ARG82714
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Product Details
内含物
| 货号 | 内含物名称 | 包装 | 温度 |
|---|---|---|---|
| ARG82714-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG82714-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG82714-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG82714-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82714-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82714-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82714-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82714-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG82714-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG82714-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG82714-011 | Plate sealer | 4 strips | Room temperature |
概述
| 产品描述 | ARG82714 Human PZP / Pregnancy zone protein ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human PZP / Pregnancy zone protein in serum, plasma (EDTA, heparin) and cell culture supernatants. |
|---|---|
| 反应物种 | Hu |
| 应用 | ELISA |
| 靶点名称 | PZP / Pregnancy zone protein |
| 偶联标记 | HRP |
| 偶联标记说明 | Substrate: TMB and read at 450 nm. |
| 灵敏度 | 78 pg/ml |
| 样品类型 | Serum, plasma (EDTA, heparin) and cell culture supernatants. |
| 标准范围 | 156 - 10000 pg/ml |
| 样本量 | 100 µl |
| 精确 | Intra-Assay CV: 5.6% Inter-Assay CV: 6.4% |
| 別名 | CPAMD6 |
应用说明
| 检测时间 | ~ 5 hours |
|---|
属性
| 形式 | 96 well |
|---|---|
| 存放说明 | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | PZP |
| 全名 | PZP alpha-2-macroglobulin like |
| 背景介绍 | The protein encoded by this gene is highly expressed in late-pregnancy serum and is similar in structure to alpha-2-macroglobulin. The encoded protein, which acts as a homotetramer, inhibits the activity of all four classes of proteinases. This protein contains cleavage sites for several proteinases. Upon binding of a proteinase, the conformation of this protein changes to trap the proteinase, limiting its activity. This protein appears to be elevated in the sera of presymptomatic Alzheimer's disease patients. [provided by RefSeq, Dec 2016] |
| 生物功能 | Is able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase. [UniProt] |
| 产品亮点 | Related products: PZP antibodies; PZP ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
检测图片 (1)
ARG82714 Human PZP / Pregnancy zone protein ELISA Kit standard curve image
ARG82714 Human PZP / Pregnancy zone protein ELISA Kit results of a typical standard run with optical density reading at 450 nm.
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