Human QPCT(Glutaminyl-peptide cyclotransferase) ELISA Kit

Key features and details

Sensitivity:0.094ng/ml
Detection Range: 0.156-10ng/ml
Sample Types: Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples
Species:Specifically binds with QPCT , no obvious cross reaction with other analogues.
Detection Method: Sandwich ELISA, Double Antibody
Target:QPCT

Brand:

CAT.NO. : ARE6631

RMB Please choose

Size:

96T

Trail, Bulk size or Custom requests Please contact us

*产品价格可能会有所调整，请以品牌方官网实时更新的价格为准，以确保准确性。

Product Details

Background

This kit is based on Double antibody-Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti QPCT antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody. Then, it binds with QPCT bound to precoated antibody. Wash unbound components and add HRP-Streptavidin Conjugate (SABC). Wash unbound components again and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of QPCT in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value.

Overview

Alias	Epiphycan ELISA Kit, Dermatan sulfate proteoglycan 3 ELISA Kit, Proteoglycan-Lb ELISA Kit, PG-Lb ELISA Kit, Small chondroitin/dermatan sulfate proteoglycan ELISA Kit, EPYC ELISA Kit, DSPG3 ELISA Kit, PGLB ELISA Kit, SLRR3B ELISA Kit
Size	96T
Species	Human
UniProt ID	Q16769
Sample Type	Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples
Detection Method	Sandwich ELISA, Double Antibody
Detection Wavelength	OD450
Reaction Duration	4 hours
Range	0.156-10ng/ml
Sensitivity	0.094ng/ml
Specificity	Specifically binds with QPCT, no obvious cross reaction with other analogues.

Standard Curve

This product is detected by QC department and meets performance required in the manual. (Laboratory Humidity: 20%-60%; Temperature: 18°C -25°C; Equilibrate TMB substrate to 37°C before staining. After adding into the ELISA wells, incubate for 15min at 37°C in dark.)

Due to different assay environments and operations, assay data below and standard curve are provided for reference. Experimenters should establish standard curve according to their own assay.

STD.(ng/ml)	OD-1	OD-2	Average
0	0.096	0.101	0.098
0.156	0.169	0.177	0.172
0.312	0.242	0.254	0.247
0.625	0.372	0.391	0.38
1.25	0.611	0.642	0.623
2.5	1.173	1.233	1.197
5	1.753	1.843	1.789
10	2.121	2.229	2.164

Recovery

Add a certain amount of QPCT into the sample. Calculate the recovery by comparing the measured value with the expected amount of QPCT in the sample.

Sample Type	Recovery Range(%)	Average(%)
serum(n=10)	86-104	94
EDTA plasma(n=10)	85-99	94
Heparin plasma(n=10)	86-100	91

Linearity

Dilute the sample with a certain amount of QPCT at 1:2, 1:4 and 1:8 to get the recovery range.

Sample Type	1:2	1:4	1:8
serum(n=10)	92-105%	92-100%	91-100%
EDTA plasma(n=10)	89-95%	85-101%	91-100%
Heparin plasma(n=10)	91-105%	85-98%	84-101%

Precision(%)

Intra-assay Precision: samples with low, medium and high concentration are tested 20 times on the same plate.

Inter-assay Precision: samples with low, medium and high concentration are tested 20 times on three different plates.

Item	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (ng/ml)	0.29	1.27	5.04	0.29	1.24	5.09
Standard deviation	0.02	0.08	0.29	0.02	0.07	0.2
CV(%)	6.24	5.92	5.72	5.89	5.34	4.02

Stability

Perform the stability test for the sealed kit at 37°C and 2-8°C and get relevant data.

ELISA kit(n=5)	37°C for 1 month	2-8°C for 6 months
Average(%)	80	95-100

Storage

Shipped and store at 2-8°C for 6 month.

Research Use Only

For Research Use Only. Not for use in diagnostic procedures.

New Products

Human Beta Amyloid 1-40 Antibody Pair

Beta Amyloid 1-40 Antibody

Human Tau antibody pair (ARA983/ARA958)

Human Tau antibody pair (ARA6837/ARA958)

Human Tau antibody pair (ARA958/ARA971)

Human pTau205 antibody pair (ARA990/ARA971)