Human Ret ELISA Kit
Key features and details
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- 偶联标记:
- 偶联标记说明:
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CAT.NO. : ARG82715
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Product Details
内含物
| 货号 | 内含物名称 | 包装 | 温度 |
|---|---|---|---|
| ARG82715-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG82715-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG82715-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG82715-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82715-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82715-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82715-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82715-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG82715-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG82715-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG82715-011 | Plate sealer | 4 strips | Room temperature |
概述
| 产品描述 | ARG82715 Human Ret ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human Ret in serum, plasma (heparin) and cell culture supernatants. |
|---|---|
| 反应物种 | Hu |
| 应用 | ELISA |
| 靶点名称 | Ret |
| 偶联标记 | HRP |
| 偶联标记说明 | Substrate: TMB and read at 450 nm. |
| 灵敏度 | 47 pg/ml |
| 样品类型 | Serum, plasma (heparin) and cell culture supernatants. |
| 标准范围 | 93.8 - 6000 pg/ml |
| 样本量 | 100 µl |
| 精确 | Intra-Assay CV: 5.0% Inter-Assay CV: 6.8% |
| 別名 | RET51; CDHF12; HSCR1; Proto-oncogene c-Ret; PTC; Proto-oncogene tyrosine-protein kinase receptor Ret; RET-ELE1; CDHR16; MEN2B; MEN2A; MTC1; EC 2.7.10.1; Cadherin family member 12 |
应用说明
| 检测时间 | ~ 5 hours |
|---|
属性
| 形式 | 96 well |
|---|---|
| 存放说明 | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | Swiss-port # P07949 Human Proto-oncogene tyrosine-protein kinase receptor Ret |
|---|---|
| 基因名称 | RET |
| 全名 | ret proto-oncogene |
| 背景介绍 | This gene, a member of the cadherin superfamily, encodes one of the receptor tyrosine kinases, which are cell-surface molecules that transduce signals for cell growth and differentiation. This gene plays a crucial role in neural crest development, and it can undergo oncogenic activation in vivo and in vitro by cytogenetic rearrangement. Mutations in this gene are associated with the disorders multiple endocrine neoplasia, type IIA, multiple endocrine neoplasia, type IIB, Hirschsprung disease, and medullary thyroid carcinoma. Two transcript variants encoding different isoforms have been found for this gene. Additional transcript variants have been described but their biological validity has not been confirmed. [provided by RefSeq, Jul 2008] |
| 生物功能 | Receptor tyrosine-protein kinase involved in numerous cellular mechanisms including cell proliferation, neuronal navigation, cell migration, and cell differentiation upon binding with glial cell derived neurotrophic factor family ligands. Phosphorylates PTK2/FAK1. Regulates both cell death/survival balance and positional information. Required for the molecular mechanisms orchestration during intestine organogenesis; involved in the development of enteric nervous system and renal organogenesis during embryonic life, and promotes the formation of Peyer's patch-like structures, a major component of the gut-associated lymphoid tissue. Modulates cell adhesion via its cleavage by caspase in sympathetic neurons and mediates cell migration in an integrin (e.g. ITGB1 and ITGB3)-dependent manner. Involved in the development of the neural crest. Active in the absence of ligand, triggering apoptosis through a mechanism that requires receptor intracellular caspase cleavage. Acts as a dependence receptor; in the presence of the ligand GDNF in somatotrophs (within pituitary), promotes survival and down regulates growth hormone (GH) production, but triggers apoptosis in absence of GDNF. Regulates nociceptor survival and size. Triggers the differentiation of rapidly adapting (RA) mechanoreceptors. Mediator of several diseases such as neuroendocrine cancers; these diseases are characterized by aberrant integrins-regulated cell migration. [UniProt] |
| 细胞定位 | Cell membrane; Single-pass type I membrane protein. Endosome membrane; Single-pass type I membrane protein. [UniProt] |
| 产品亮点 | Related products: Ret antibodies; Ret ELISA Kits; Ret Duos / Panels; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| 翻译后修饰 | Autophosphorylated on C-terminal tyrosine residues upon ligand stimulation. Dephosphorylated by PTPRJ on Tyr-905, Tyr-1015 and Tyr-1062. Proteolytically cleaved by caspase-3. The soluble RET kinase fragment is able to induce cell death. The extracellular cell-membrane anchored RET cadherin fragment accelerates cell adhesion in sympathetic neurons. [UniProt] |
检测图片 (1)
ARG82715 Human Ret ELISA Kit standard curve image
ARG82715 Human Ret ELISA Kit results of a typical standard run with optical density reading at 450 nm.
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