Human SCF/KITLG ELISA Kit
Key features and details
- Specification:
- Sensitivity:
- Standard Curve Range:
- Standard Curve Gradient:
- Number of Incubations:
- Sample Volume:
- Assay type:
- Operation Duration:
-
Brand:
CAT.NO. : AEH0174
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Size:
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Product Details
Background
Stem cell factor (SCF), also known as c-kit ligand (KL), mast cell growth factor (MGF), and steel factor (SLF), is a widely expressed 28 - 40 kDa type I transmembrane glycoprotein. It promotes the survival, differentiation, and mobilization of multiple cell types including myeloid, erythroid, megakaryocytic, lymphoid, germ cell, and melanocyte progenitors. SCF is a primary growth and activation factor for mast cells and eosinophils. Mature mouse SCF consists of a 189 amino acid (aa) extracellular domain (ECD), a 23 aa transmembrane segment, and a 36 aa cytoplasmic tail. The ECD shows both N-linked and O-linked glycosylation. Proteolytic cleavage at two alternate sites in the extracellular juxtamembrane region releases a 25 kDa soluble molecule which is comparable to the only form produced by Steel-dickie mutant mice. An alternately spliced isoform of mouse SCF lacks 28 aa that encompasses the primary proteolytic recognition site. Within the ECD of the short isoform (corresponding to this recombinant protein), mouse SCF shares 93% aa sequence identity with rat SCF and 72% - 75% with canine, feline, and human SCF. Rat SCF is active on mouse and human cells, but human SCF is only weakly active on mouse cells. Noncovalent dimers of transmembrane or soluble SCF interact with the receptor tyrosine kinase SCF R/c-kit to trigger receptor dimerization and signaling. SCF assists in the recovery of cardiac function following myocardial infarction by increasing the number of cardiomyocytes and vascular channels.
Typical data
|
pg/ml |
O.D. |
Average |
Corrected |
|
|
0.00 |
0.0153 |
0.0131 |
0.0142 |
|
|
6.86 |
0.0424 |
0.0400 |
0.0412 |
0.0270 |
|
20.58 |
0.0919 |
0.0914 |
0.0917 |
0.0775 |
|
61.73 |
0.2355 |
0.2313 |
0.2334 |
0.2192 |
|
185.19 |
0.6456 |
0.6603 |
0.6530 |
0.6388 |
|
555.56 |
1.6390 |
1.6610 |
1.6500 |
1.6358 |
|
1666.67 |
3.0210 |
3.1300 |
3.0755 |
3.0613 |
|
5000.00 |
3.9070 |
4.0151 |
3.9611 |
3.9469 |
Precision
|
Intra-assay Precision |
Inter-assay Precision |
|||||
|
Sample Number |
S1 |
S2 |
S3 |
S1 |
S2 |
S3 |
|
22 |
22 |
22 |
6 |
6 |
6 |
|
|
Average(pg/ml) |
107.7 |
508.8 |
1485.5 |
112.3 |
503.0 |
1608.2 |
|
Standard Deviation |
6.1 |
27.0 |
105.1 |
7.0 |
30.9 |
115.0 |
|
Coefficient of Variation(%) |
5.6 |
5.3 |
7.1 |
6.2 |
6.1 |
7.2 |
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human SCF/KITLG into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 71% to 90% with an overall mean recovery of 82%.
The recovery ranged from 71% to 90% with an overall mean recovery of 82%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | 1574.28-4366.78 | 100 | 2624.25 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of SCF/KITLG in this assay. No medical histories were available for the donors.
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