Human SFRP5 ELISA Kit
Key features and details
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- 反应物种:
- 应用:
- 特异性:
- 靶点名称:
- 偶联标记:
- 偶联标记说明:
- 灵敏度:
- 样品类型:
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Brand:
CAT.NO. : ARG81585
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Product Details
内含物
| 货号 | 内含物名称 | 包装 | 温度 |
|---|---|---|---|
| ARG81585-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG81585-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG81585-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG81585-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81585-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81585-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG81585-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG81585-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG81585-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG81585-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG81585-011 | Plate sealer | 4 strips | Room temperature |
概述
| 产品描述 | ARG81585 Human SFRP5 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human SFRP5 in serum, plasma (heparin, EDTA) and cell culture supernatants. |
|---|---|
| 反应物种 | Hu |
| 应用 | ELISA |
| 特异性 | There is no detectable cross-reactivity with other relevant proteins. |
| 靶点名称 | SFRP5 |
| 偶联标记 | HRP |
| 偶联标记说明 | Substrate: TMB and read at 450 nm. |
| 灵敏度 | 78 pg/ml |
| 样品类型 | Serum, plasma (heparin, EDTA) and cell culture supernatants. |
| 标准范围 | 156 - 10000 pg/ml |
| 样本量 | 100 µl |
| 精确 | Intra-Assay CV: 5.6% Inter-Assay CV: 6.7% |
| 別名 | Secreted apoptosis-related protein 3; Secreted frizzled-related protein 5; SARP3; Frizzled-related protein 1b; SARP-3; FRP-1b; sFRP-5 |
应用说明
| 检测时间 | ~ 5 hours |
|---|
属性
| 形式 | 96 well |
|---|---|
| 存放说明 | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | Swiss-port # Q5T4F7 Human Secreted frizzled-related protein 5 |
|---|---|
| 基因名称 | SFRP5 |
| 全名 | secreted frizzled-related protein 5 |
| 背景介绍 | Secreted frizzled-related protein 5 (SFRP5) is a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. SFRPs act as soluble modulators of Wnt signaling. SFRP5 and SFRP1 may be involved in determining the polarity of photoreceptor cells in the retina. SFRP5 is highly expressed in the retinal pigment epithelium, and moderately expressed in the pancreas. [provided by RefSeq, Jul 2008] |
| 生物功能 | Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types. SFRP5 may be involved in determining the polarity of photoreceptor, and perhaps, other cells in the retina. [UniProt] |
| 产品亮点 | Related products: New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
检测图片 (1)
ARG81585 Human SFRP5 ELISA Kit standard curve image
ARG81585 Human SFRP5 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
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