Human SMPD1 / Acid Sphingomyelinase ELISA Kit
Key features and details
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Brand:
CAT.NO. : ARG82482
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Product Details
内含物
| 货号 | 内含物名称 | 包装 | 温度 |
|---|---|---|---|
| ARG82482-001 | Antibody-coated microplate | 8 X 12 strips | 4°C. Unused strips should be sealed tightly in the air-tight pouch. |
| ARG82482-002 | Standard | 2 X 10 ng/vial | 4°C |
| ARG82482-003 | Standard/Sample diluent | 30 ml (Ready to use) | 4°C |
| ARG82482-004 | Antibody conjugate concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82482-005 | Antibody diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82482-006 | HRP-Streptavidin concentrate (100X) | 1 vial (100 µl) | 4°C |
| ARG82482-007 | HRP-Streptavidin diluent buffer | 12 ml (Ready to use) | 4°C |
| ARG82482-008 | 25X Wash buffer | 20 ml | 4°C |
| ARG82482-009 | TMB substrate | 10 ml (Ready to use) | 4°C (Protect from light) |
| ARG82482-010 | STOP solution | 10 ml (Ready to use) | 4°C |
| ARG82482-011 | Plate sealer | 4 strips | Room temperature |
概述
| 产品描述 | ARG82482 Human SMPD1 / Acid Sphingomyelinase ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human SMPD1 / Acid Sphingomyelinase in serum, plasma (heparin) and cell culture supernatants. |
|---|---|
| 反应物种 | Hu |
| 应用 | ELISA |
| 靶点名称 | SMPD1 / Acid Sphingomyelinase |
| 偶联标记 | HRP |
| 偶联标记说明 | Substrate: TMB and read at 450 nm. |
| 灵敏度 | 10 pg/ml |
| 样品类型 | Serum, plasma (heparin) and cell culture supernatants. |
| 标准范围 | 15.6 - 1000 pg/ml |
| 样本量 | 100 µl |
| 精确 | Intra-Assay CV: 4.7% Inter-Assay CV: 6.5% |
| 別名 | aSMase; EC 3.1.4.12; NPD; ASMASE; Sphingomyelin phosphodiesterase; Acid sphingomyelinase; ASM |
应用说明
| 检测时间 | ~ 5 hours |
|---|
属性
| 形式 | 96 well |
|---|---|
| 存放说明 | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | SMPD1 |
| 全名 | sphingomyelin phosphodiesterase 1, acid lysosomal |
| 背景介绍 | The protein encoded by this gene is a lysosomal acid sphingomyelinase that converts sphingomyelin to ceramide. The encoded protein also has phospholipase C activity. Defects in this gene are a cause of Niemann-Pick disease type A (NPA) and Niemann-Pick disease type B (NPB). Multiple transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2010] |
| 生物功能 | Converts sphingomyelin to ceramide. Also has phospholipase C activities toward 1,2-diacylglycerolphosphocholine and 1,2-diacylglycerolphosphoglycerol. Isoform 2 and isoform 3 have lost catalytic activity. [UniProt] |
| 细胞定位 | Lysosome. Secreted. [UniProt] |
| 产品亮点 | Related products: SMPD1 / Acid Sphingomyelinase antibodies; SMPD1 / Acid Sphingomyelinase ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
检测图片 (1)
ARG82482 Human SMPD1 / Acid Sphingomyelinase ELISA Kit standard curve image
ARG82482 Human SMPD1 / Acid Sphingomyelinase ELISA Kit results of a typical standard run with optical density reading at 450 nm.
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