Human TDGF1 / Cripto 1 recombinant protein (His-tagged, C-ter)
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CAT.NO. : ARG70273
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Product Details
概述
| 产品描述 | HEK293 expressed, His-tagged (C-ter) Human TDGF1 / Cripto 1 recombinant protein. |
|---|---|
| 反应物种 | Hu |
| 应用 | Binding, SDS-PAGE |
| 靶点名称 | TDGF1 / Cripto 1 |
| 物种 | Human |
| A.A. 序列 | Leu31 - Thr172 of Human TDGF1 / Cripto 1 (NP_003203) with 6X His tag at the C-terminus. |
| 表达系统 | HEK293 |
| 別名 | CRGF; Teratocarcinoma-derived growth factor 1; Cripto-1 growth factor; CR; CRIPTO; Epidermal growth factor-like cripto protein CR1 |
应用说明
| 应用说明 | Binding activity test: Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human Cripto at 2 µg/ml (100µl/well) can bind Recombinant Human ALK4 with a linear range of 0.15-151 ng/ml. |
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属性
| 形式 | Powder |
|---|---|
| 纯化说明 | 0.22 µm filter sterilized. Endotoxin level is less than 0.1 EU/µg of the protein, as determined by the LAL test. |
| 纯度 | > 95% (by SDS-PAGE) |
| 缓冲液 | PBS (pH 7.4) |
| 复溶 | Reconstitute to a concentration of 0.1 - 0.5 mg/ml in sterile distilled water. |
| 存放说明 | For long term, lyophilized protein should be stored at -20°C or -80°C. After reconstitution, aliquot and store at -20°C for up to one month, at 2-8°C for up to one week. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | Swiss-port # P13385 Human Teratocarcinoma-derived growth factor 1 |
|---|---|
| 基因名称 | TDGF1 |
| 全名 | teratocarcinoma-derived growth factor 1 |
| 背景介绍 | This gene encodes an epidermal growth factor-related protein that contains a cripto, FRL-1, and cryptic domain. The encoded protein is an extracellular, membrane-bound signaling protein that plays an essential role in embryonic development and tumor growth. Mutations in this gene are associated with forebrain defects. Pseudogenes of this gene are found on chromosomes 2, 3, 6, 8, 19 and X. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Mar 2010] |
| 生物功能 | GPI-anchored cell membrane protein involved in Nodal signaling. Cell-associated TDGF1 acts as a Nodal coreceptor in cis. Shedding of TDGF1 by TMEM8A modulates Nodal signaling by allowing soluble TDGF1 to act as a Nodal coreceptor on other cells (PubMed:27881714). Could play a role in the determination of the epiblastic cells that subsequently give rise to the mesoderm (PubMed:11909953). [UniProt] |
| 细胞定位 | Cell membrane; Lipid-anchor, GPI-anchor. Secreted. Note=Released from the cell membrane by GPI cleavage. [UniProt] |
| 预测分子量 | 21 kDa |
检测图片 (1)
ARG70273 Human TDGF1 / Cripto 1 recombinant protein (His-tagged, C-ter) SDS-PAGE image
SDS-PAGE analysis of ARG70273 Human TDGF1 / Cripto 1 recombinant protein (His-tagged, C-ter).
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