Human TGF alpha ELISA Kit
Key features and details
- Specification:
- Sensitivity:
- Standard Curve Range:
- Standard Curve Gradient:
- Number of Incubations:
- Sample Volume:
- Assay type:
- Operation Duration:
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Brand:
CAT.NO. : AEH0059
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Product Details
Background
TGF-alpha is a member of the EGF family of cytokines that are synthesized as transmembrane precursors with soluble forms released by proteolytic cleavage. Membrane-bound proTGF-alpha is biologically active and plays a role in mediation of cell-cell adhesion and in juxtacrine stimulation of adjacent cells. Expression of TGF-alpha is widespread in tumors and transformed cells. TGF-alpha is also expressed in normal tissues during embryogenesis and in adult tissues, including pituitary, brain, keratinocytes and macrophages. TGF-alpha binds and activates the EGF receptor. It induces fibroblast proliferation, epithelial development, keratinocyte migration, and angiogenesis.
Typical data
| pg/ml | O.D. | Average | Corrected | |
| 0.00 | 0.0422 | 0.0382 | 0.0402 | |
| 4.12 | 0.0471 | 0.0527 | 0.0499 | 0.0439 |
| 12.35 | 0.0726 | 0.0712 | 0.0719 | 0.0659 |
| 37.04 | 0.1285 | 0.1285 | 0.1285 | 0.1225 |
| 111.11 | 0.3091 | 0.3135 | 0.3113 | 0.3053 |
| 333.33 | 0.7566 | 0.7792 | 0.7679 | 0.7619 |
| 1000.00 | 1.7560 | 1.6690 | 1.7125 | 1.7065 |
| 3000.00 | 3.1350 | 3.1530 | 3.1440 | 3.1380 |
Precision
| Intra-assay Precision | Inter-assay Precision | |||||
| Sample Number | S1 | S2 | S3 | S1 | S2 | S3 |
| 22 | 22 | 22 | 6 | 6 | 6 | |
| Average(pg/ml) | 59.6 | 296.2 | 1122.0 | 58.2 | 320.6 | 1180.8 |
| Standard Deviation | 2.7 | 20.8 | 74.4 | 2.3 | 16.3 | 40.8 |
| Coefficient of Variation(%) | 4.5 | 7.0 | 6.6 | 4.0 | 5.1 | 3.5 |
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human TGF-α into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 104% to 126% with an overall mean recovery of 114%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
| Serum | 30 | n.d.-25.28 | 50 | 9.57 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of TGF-α in this assay. No medical histories were available for the donors.
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