Human Thioredoxin-1/Trx-1 ELISA Kit
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CAT.NO. : ARG80944
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Product Details
内含物
| 货号 | 内含物名称 | 包装 | 温度 |
|---|---|---|---|
| ARG80944-001 | Antibody-coated microplate | 96 well | 4°C |
| ARG80944-002 | Standard (Lyophilized) | 3 X 2 ng/vial | 4°C |
| ARG80944-003 | Sample Diluent | 20 ml | 4°C |
| ARG80944-004 | Biotinylated Trx1 antibody (30X) | 1 vial | 4°C |
| ARG80944-005 | Antibody Diluent | 16 ml | 4°C |
| ARG80944-006 | Streptavidin-HRP | 1 vial | 4°C |
| ARG80944-007 | Streptavidin-HRP Diluent | 16 ml | 4°C |
| ARG80944-008 | TMB Substrate | 12 ml | 4°C (Protect from light) |
| ARG80944-009 | 20X Wash buffer | 50 ml | 4°C |
| ARG80944-010 | Plate sealer | 4 strips | Room temperature |
| ARG80944-011 | STOP solution | 12 ml | 4°C |
概述
| 产品描述 | ARG80944 Human Thioredoxin-1/Trx-1 ELISA Kit is a Enzyme Immunoassay kit for the quantification of Human Thioredoxin-1/Trx-1 in Serum, Plasma and Cell culture supernatants. |
|---|---|
| 反应物种 | Hu |
| 应用 | ELISA |
| 靶点名称 | TRX1 / Thioredoxin 1 |
| 偶联标记 | HRP |
| 灵敏度 | 62.5 pg/ml |
| 样品类型 | Serum, Plasma and Cell culture supernatants |
| 标准范围 | 125 - 8000 pg/ml |
| 样本量 | 100 μl |
| 別名 | ATL-derived factor; TRX1; SASP; Trx; ADF; TRX; Surface-associated sulphydryl protein; TRDX; Thioredoxin |
应用说明
| 检测时间 | ~200 min |
|---|
属性
| 形式 | 96 well |
|---|---|
| 存放说明 | Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. |
| 注意事项 | For laboratory research only, not for drug, diagnostic or other use. |
生物信息
| 数据库连接 | |
|---|---|
| 基因名称 | TXN |
| 全名 | thioredoxin |
| 背景介绍 | The protein encoded by this gene acts as a homodimer and is involved in many redox reactions. The encoded protein is active in the reversible S-nitrosylation of cysteines in certain proteins, which is part of the response to intracellular nitric oxide. This protein is found in the cytoplasm. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011] |
| 生物功能 | Participates in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide and catalyzes dithiol-disulfide exchange reactions. Plays a role in the reversible S-nitrosylation of cysteine residues in target proteins, and thereby contributes to the response to intracellular nitric oxide. Nitrosylates the active site Cys of CASP3 in response to nitric oxide (NO), and thereby inhibits caspase-3 activity. Induces the FOS/JUN AP-1 DNA-binding activity in ionizing radiation (IR) cells through its oxidation/reduction status and stimulates AP-1 transcriptional activity. ADF augments the expression of the interleukin-2 receptor TAC (IL2R/P55). [UniProt] |
| 产品亮点 | Related products: TRX1 antibodies; TRX1 ELISA Kits; New ELISA data calculation tool: Simplify the ELISA analysis by GainData |
| 研究领域 | Metabolism kit; Signaling Transduction kit |
| 翻译后修饰 | In the fully reduced protein, both Cys-69 and Cys-73 are nitrosylated in response to nitric oxide (NO). When two disulfide bonds are present in the protein, only Cys-73 is nitrosylated. Cys-73 can serve as donor for nitrosylation of target proteins. In case of infection, ubiquitinated by S.typhimurium protein slrP, leading to its degradation. |
检测图片 (1)
ARG80944 Human Thioredoxin-1/Trx-1 ELISA Kit standard curve image
ARG80944 Human Thioredoxin-1/Trx-1 ELISA Kit results of a typical standard run with optical density reading at 450 nm.
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