Human TNF beta/Lymphotoxin alpha ELISA Kit
Key features and details
- Specification:
- Sensitivity:
- Standard Curve Range:
- Standard Curve Gradient:
- Number of Incubations:
- Sample Volume:
- Assay type:
- Operation Duration:
-
Brand:
CAT.NO. : AEH0181
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Size:
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Product Details
Background
Lymphotoxin-alpha (LT-alpha ), also known as Tumor Necrosis Factor-beta (TNF-beta ), is a member of the TNF Superfamily. Human LT-alpha /TNF-beta is a 22 kDa protein that shares 73% amino acid sequence identity with mouse and rat LT-alpha /TNF-beta. Secreted LT-alpha forms homotrimers that bind and activate TNF RI/TNFRSF1A, TNF RII/TNFRSF1B, and HVEM/TNFRSF14. LT-alpha /TNF-beta also forms heterotrimers with plasma membrane-localized LT-beta to bind and activate the LT-beta R/TNFRSF3. In addition to its cytotoxic action on tumor cells, LT-alpha /TNF-beta mediates lymph node development, inflammation, and immune function. LT-alpha /TNF-beta is expressed in activated T- and B lymphocytes and contributes to autoimmune disease.
Typical data
|
pg/ml |
O.D. |
Average |
Corrected |
|
|
0.00 |
0.0139 |
0.0137 |
0.0138 |
|
|
40.96 |
0.0270 |
0.0261 |
0.0266 |
0.0128 |
|
102.40 |
0.0495 |
0.0454 |
0.0475 |
0.0337 |
|
256.00 |
0.1006 |
0.0943 |
0.0975 |
0.0837 |
|
640.00 |
0.2308 |
0.2137 |
0.2223 |
0.2085 |
|
1600.00 |
0.5287 |
0.4955 |
0.5121 |
0.4983 |
|
4000.00 |
1.2490 |
1.1550 |
1.2020 |
1.1882 |
|
10000.00 |
2.4840 |
2.4060 |
2.4450 |
2.4312 |
Precision
|
Intra-assay Precision |
Inter-assay Precision |
|||||
|
Sample Number |
S1 |
S2 |
S3 |
S1 |
S2 |
S3 |
|
22 |
22 |
22 |
6 |
6 |
6 |
|
|
Average(pg/ml) |
326.8 |
1415.9 |
4105.8 |
226.7 |
1146.4 |
3807.0 |
|
Standard Deviation |
21.7 |
86.6 |
262.9 |
8.1 |
65.9 |
68.6 |
|
Coefficient of Variation(%) |
6.6 |
6.1 |
6.4 |
3.6 |
5.7 |
1.8 |
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human TNF beta/Lymphotoxin alpha into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 92% to 117% with an overall mean recovery of 105%.
The recovery ranged from 92% to 117% with an overall mean recovery of 105%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
|---|---|---|---|---|
| Serum | 30 | n.d.-31.50 | 25 | 16.64 |
Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of TNF beta/Lymphotoxin alpha in this assay. No medical histories were available for the donors.
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