Human TNF RII/TNFRSF1B ELISA Kit
Protocol Booklet
Key features and details
- Specification:
- Sensitivity:
- Standard Curve Range:
- Standard Curve Gradient:
- Number of Incubations:
- Sample Volume:
- Assay type:
- Operation Duration:
-
Brand:
CAT.NO. : AEH0148
RMB Please choose
RMB Please choose
Size:
Trail, Bulk size or Custom requests Please contact us
*产品价格可能会有所调整，请以品牌方官网实时更新的价格为准，以确保准确性。
Product Details
Background
TNF RII (Tumor Necrosis Factor Receptor II), also known as TNFRSF1B, p75/p80, and CD120b, is a widely expressed receptor for membrane-associated TNF-alpha and Lymphotoxin-alpha. Its activation initiates pro-inflammatory and pro-survival responses via NFkB-dependent signaling pathways, although it may also induce apoptosis.
Typical data
|
pg/ml |
O.D. |
Average |
Corrected |
|
|
0.00 |
0.0125 |
0.0118 |
0.0122 |
|
|
5.49 |
0.0234 |
0.0224 |
0.0229 |
0.0108 |
|
16.46 |
0.0440 |
0.0410 |
0.0425 |
0.0304 |
|
49.38 |
0.1080 |
0.1087 |
0.1084 |
0.0962 |
|
148.15 |
0.2952 |
0.2944 |
0.2948 |
0.2827 |
|
444.44 |
0.8058 |
0.8283 |
0.8171 |
0.8049 |
|
1333.33 |
1.9330 |
1.9890 |
1.9610 |
1.9489 |
|
4000.00 |
3.6260 |
3.6600 |
3.6430 |
3.6309 |
Precision
|
|
Intra-assay Precision |
Inter-assay Precision |
||||
|
Sample Number |
S1 |
S2 |
S3 |
S1 |
S2 |
S3 |
|
22 |
22 |
22 |
6 |
6 |
6 |
|
|
Average(pg/ml) |
77.5 |
375.0 |
1222.5 |
75.9 |
372.9 |
1201.0 |
|
Standard Deviation |
4.4 |
18.1 |
69.5 |
4.2 |
16.8 |
26.2 |
|
Coefficient of Variation(%) |
5.7 |
4.8 |
5.7 |
5.5 |
4.5 |
2.2 |
Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.
Spike Recovery
The spike recovery was evaluated by spiking 3 levels of human TNF RⅡ/TNFRSF1B into health human serum sample. The un-spiked serum was used as blank in this experiment. The recovery ranged from 97% to 120% with an overall mean recovery of 115%.
Sample Values
| Sample Matrix | Sample Evaluated | Range (pg/ml) | Detectable (%) | Mean of Detectable (pg/ml) |
| Serum | 30 | 24.09-59.68 | 100 | 34.98 |
New Products
