Human Total HSP60 ELISA Kit

Key features and details

Specification: 96 Test
Sensitivity: 3.22 pg/ml (50 μl)；4.45 pg/ml (10 μl)
Standard Curve Range: 4.10~1000 pg/ml
Standard Curve Gradient: 7 Points
Number of Incubations: 2
Sample Volume: 50 μl/10 μl
Assay type: Sandwich Elisa
Operation Duration: 120min

Brand:

CAT.NO. : AEH0060

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Size:

96T

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Product Details

Background

Heat shock proteins (HSPs) are a family of highly conserved stress response proteins. Heat shock proteins function primarily as molecular chaperones by facilitating the folding of other cellular proteins, preventing protein aggregation or targeting improperly folded proteins to specific degradative pathways. HSPs are typically expressed at low levels under normal physiological conditions but are dramatically upregulated in response to cellular stress. Heat Shock Protein 60 (HSP60), also known as Chaperonin 60 (CPN60), is a mitochondrial matrix protein belonging to a highly conserved family of molecular chaperone and stress response proteins. HSP60 plays a role in stabilizing and refolding proteins in response to heat-shock or other cellular stress. Full length human HSP60 is 98% identical to mouse and rat HSP60.

Typical Data

pg/ml	O.D.		Average	Corrected
0.00	0.0310	0.0327	0.0319
4.10	0.0436	0.0462	0.0449	0.0131
10.24	0.0678	0.0691	0.0685	0.0366
25.60	0.1196	0.1236	0.1216	0.0898
64.00	0.2535	0.2621	0.2578	0.2260
160.00	0.5522	0.5783	0.5653	0.5334
400.00	1.2460	1.2700	1.2580	1.2262
1000.00	2.5840	2.5540	2.5690	2.5372

Precision

	Intra-assay Precision			Inter-assay Precision
Sample Number	S1	S2	S3	S1	S2	S3
Sample Number	22	22	22	6	6	6
Average（pg/ml）	26.6	99.0	277.0	27.8	101.2	284.9
Standard Deviation	2.3	5.6	21.7	1.8	5.3	14.5
Coefficient of Variation（%）	8.5	5.6	7.8	6.3	5.2	5.1

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays) Three samples of known concentration were tested six times on one plate to assess intra-assay precision.

Spike Recovery

The spike recovery was evaluated by spiking 3 levels of human Total HSP60 into health human serum sample. The un-spiked serum was used as blank in this experiment.
The recovery ranged from 66% to 73% with an overall mean recovery of 69%.

Sample Values

Sample

Matrix

Sample

Evaluated

Range

(ng/ml)

Detectable

Mean of Detectable

(ng/ml)

Serum

n.d.-20.90

8.98

Serum/Plasma – Thirty samples from apparently healthy volunteers were evaluated for the presence of IL-12/IL-23 p40 in this assay. No medical histories were available for the donors.

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